| 摘要: |
| 为了了解导入Bt基因是否会引起玉米对磷元素利用能力发生改变，本研究采用转基因抗虫玉米Bt 38及其背景材料郑单958（ZD 958）为试验材料，进行水培试验，设置正常供磷（1 mmol/L）和低磷（1 μmol/L）两个水平。结果表明：在相同磷浓度下，ZD 958与Bt 38的某些生物性状、生理性状显著地不同，在生物性状上，正常供磷条件下与ZD 958相比，Bt 38根长极显著降低，全展叶叶面积极显著减小，低磷条件下与ZD 958相比，Bt 38株高、根长、茎粗、叶长，叶宽、地上鲜重、地下鲜重均极显著增加，根冠比显著下降；在生理特征上，相同供磷条件下，与ZD 958 相比，抗虫玉米心叶、全展叶叶绿素含量及Chla/Chlb均有显著性变化，抗虫玉米不同部位可溶性糖、淀粉含量、激素含量亦有显著性变化。此外，全展叶中可溶性蛋白含量和抗氧化物酶活性也有显著性变化。综上，Bt基因的导入改变了ZD 958苗期对磷元素的利用能力，从而使ZD 958与Bt 38在不同磷环境下表现出不同的生存能力。 |
| 关键词: 玉米 Bt 38 郑单958 抗虫 低磷胁迫 CrylAc |
| DOI：10.11841/j.issn.1007-4333.2014.03.09 |
| 投稿时间：2013-08-14 |
| 基金项目:农业部转基因生物新品种培育重大专项（2013ZX08011-023） |
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| Effect of low phosphorus stress on insect-resistant maize at seedling stages |
|
SU He, NIU Xiao-xue, CHEN Xiao-wen, SHEN Ting-ting, DONG Xue-hui
|
| (College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China) |
| Abstract: |
| In order to explore the difference of phosphorus(P)use efficiency between Bt corn and Non-Bt corn, we take Bt 38 (Bt maize) and the background maize ZD 958 (NON-Bt maize) as experimental materials, using hydroponics at two P concentration levels (1 mmol/L, 1 μmol/L) to test the effect.The result showed that some biological traits and physical signs presented significant difference even under the same P concentrations between Bt 38 and ZD 958.Among the biological traits, root length, full-developed leave area from Bt corn decreased markedly under normal P concentration compared with ZD 958.Furthermore, plant height, root length, Stem diameter, leaf length, leaf width, shoot fresh weight, root fresh weight all increased dramatically, while root/shoot ratio decreased significantly.Additionally, among physical signs, compared with Bt 38 vs ZD 958, Chlorophyll content and Chla/Chlb of unfully-developed and fully-developed leaves, solvable sugars and starch content as well as plant hormone content among different part of maize changed markedly.Moreover, solvable proteins content, activities of anti-oxidant enzymes changed significantly under different P concentration treatments.In a word, expression of Cry1Ac affected both biological and physical traits in Bt corn, which lead to diverse viabilities under the same P concentrations. |
| Key words: maize Bt 38 ZD 958 insect-resistant low phosphorus stress Cry1Ac |
