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| 山东猪产业链中大肠杆菌耐药性及传递的研究 |
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胡明1, 白华1, 齐静1, 骆延波1, 蔡亚娜1,2, 吴聪明3, 刘玉庆1,2
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| (1.山东省农业科学院 畜牧兽医研究所/山东省动物疫病防治与繁育重点试验室, 济南 250100;2.青岛农业大学 动物科技学院, 山东 青岛 266109;3.中国农业大学 动物医学院, 北京 100193) |
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| 摘要: |
| 为比较养殖区猪“养殖-屠宰-销售”产业链各个环节中大肠杆菌的耐药性状况，及其在动物与动物、与人之间传递的情况，采集猪产业链中多个环节的样品，分离大肠杆菌150株，用肉汤稀释法检测细菌对常用药物的最小抑菌浓度（Minimum inhibitory concentration，MIC），并对所有菌株进行脉冲场凝胶电泳（Pulsed field gel electrophoresis，PFGE）分型分析。研究结果表明养殖环节中（包括饲养员）细菌耐药程度普遍较高且呈现多重耐药性，屠宰、加工和销售过程中细菌耐药程度较低；在仔猪、哺乳母猪和怀孕母猪之间，及保育猪和饲养员之间存在PFGE型高度相似的菌株，而PFGE型相似菌株的耐药性则存在不同程度的差异。说明良好的屠宰条件在一定程度上能够阻断养殖环节形成的高耐药性菌株的传递，耐药性大肠杆菌菌株可以在密切接触的人和动物之间传播。评估猪产业链中动物源条件致病菌及耐药性的转移扩散风险，对食品安全和公共卫生具有一定参考价值。 |
| 关键词: 猪 产业链 大肠杆菌 耐药性 脉冲场凝胶电泳 |
| DOI：10.11841/j.issn.1007-4333.2014.02.23 |
| 投稿时间：2013-06-19 |
| 基金项目:公益性行业（农业）科研专项（200903055，201203040）；山东省优秀中青年科学家科研奖励基金（BS2010NY026） |
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| Antibiotic resistance and its’ transmission of E.coli from the swine production chain in Shandong Province |
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HU Ming1, BAI Hua1, QI Jing1, LUO Yan-bo1, CAI Ya-na1,2, WU Cong-ming3, LIU Yu-qing1,2
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| (1.Institute of Animal Science and Veterinary Medicine/Key Laboratory of Animal Disease Control & Breeding, Shandong Academy of Agricultural Sciences, Jinan 250100, China;2.College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China;3.College of Veterinary Medicine, China Agricultural University, Beijing 100193, China) |
| Abstract: |
| To understand the bacterial resistance to antibiotics and its transmission between animals,or between animals and people,150 strains of Escherichia coli were isolated and identified from the swine production chain,including breeding,butchering and selling in Shandong Province.The antimicrobial susceptibility of these isolates was tested by using a broth dilution method to determine the minimum inhibitory concentration of commonly used drugs.The pulsed field gel electrophoresis (PFGE)types were applied to identify the homogeneity DNA types of all strains.The results showed that the bacterial resistance increased in the breeding links including that of isolates from corresponding animal keepers,while decreased in the links of butchering and selling.With PFGE analysis,highly homogeneous isolates were detected among piglets,lactating sows,pregnant sows and keepers,while their resistant profiles were different to a certain extent.Good manufacturing practice of slaughterhouse could keep meat away from resistant intestinal flora to a certain degree.The resistance of E.coli could transmit between swines and growers closely touched.The results would contribute to evaluating potential risk factors of the animal pathogens transmission and drug resistance in the food chain and public health. |
| Key words: swine swine production chain Escherichia coli bacterial resistance PFGE |
