| 摘要: |
| 为更好地指导矿区进行受损土地的微生物复垦，减少传统监测方法对植物的损害，需要运用快速有效的方法来监测菌根作用下植物的生长状况。对不同生长期的大豆叶片光谱、叶绿素含量、菌根侵染率和菌丝密度测定分析 ，结果表明：各生长期接菌组叶绿素含量均高于同期对照，其叶绿素含量与侵染率间具有显著正相关性，且叶片光谱反射率在550 nm处显著低于对照组。同时根据叶片光谱可见光区域叶绿素敏感波段选择植被指数，然后建立以植被指数为变量的线性模型反演叶绿素含量，结果发现植被指数MCARI反演叶绿素含量的精度及稳定性（R2=0.67）都优于其他指数，其线性模型反演绝对误差为2.504，相对误差为6.57%。该研究结果说明高光谱技术是估测菌根作用下大豆叶绿素含量的一种简单、快捷和无损的有效方法，也为未来利用高光谱数据评估微生物对植物生长的促进作用提供理论与方法支持。 |
| 关键词: 大豆 菌根作用 高光谱反射率 光谱差异 高光谱指数 |
| DOI：10.11841/j.issn.1007-4333.2014.02.18 |
| 投稿时间：2013-08-30 |
| 基金项目:十二五科技支撑计划（2012BAC10B03） |
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| Hyperspetral characteristics and estimation for chlorophyll content of soybean inoculated with AMF |
|
CHEN Shu-lin, BI Yin-li, QI Li-shuai, FENG Yan-bo, ZHANG Yan-xu, WANG Jin
|
| (College of Geoscience and Surveying Engineering, China University of Mining & Technology, Beijing 100083, China) |
| Abstract: |
| In order to guide the microbial land reclamation in coal mining,it is necessary to look for a quick and non-destructive way and thus obtain growth information of plants inoculated with mycorrhiza.The measured parameters included hyperspetral spectra,inocurated rate(IR),hyphal density and chlorophyll content (SPAD values) of soybeans with or without inoculation of arbuscular mycorrhiza fungi (AMF).The results showed that the chlorophyll content of soybean leaf inoculated with AMF was higher than that of the control in each period.The leaf spectra reflectance of the inoculation was apparently lower than that of the control in 550 nm.There was a positive correlation between IR and the chlorophyll content.Smoothing the leaf spectra,the ratio indices were selected by using the sensitive bands for chlorophyll in VIS.The linear models for estimating chlorophyll content were built by using ratio indices as variables.The analysis results indicated that the inversion precision and the stability of chlorophyll content calculated from ratio index MCARI were better than those from other index.The absolute error was 2.504,the relative error 6.57%.The results could not only provide with a simple,quick and non-destructive way for estimating the leaf chlorophyll content of soybean,but also supply with theories and methods for assessing the enhancement of plant growth resulted from AMF by using hyperspectral data. |
| Key words: soybean mycorrhizal effect hyperspectral reflectance spectral differentiation vegetation indices |
