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鸡粪锯末好氧堆肥过程中硝化细菌动态变化
陈雅娟, 霍培书, 韩卉, 李季
0
(中国农业大学 资源与环境学院, 北京 100193)
摘要:
用鸡粪与锯末为原料进行高温好氧堆肥试验,研究不同初始含水率及堆肥方式下鸡粪锯末堆肥中铵态氮及硝化细菌动态变化,以期减少堆肥过程中氮素的损失,为优化堆肥提供理论依据。结果表明:1)硝化细菌存在于整个堆肥过程,且在堆肥腐熟期硝化细菌群落结构均发生了较大的变化。2)铵态氮浓度与硝化细菌群落物种丰富度与稳定性有关,可能是铵态氮会诱发硝化细菌生长。3)含水率是导致硝化细菌群落结构变化的关键因素。4)通过分析T-RF150、169和343 bp对堆肥环境的适应性较强,且多数属于是不可培养的细菌菌属。5)通过分析可知T3处理有助于硝化细菌群落生长与稳定。
关键词:  鸡粪  堆肥  铵态氮  硝化细菌
DOI:10.11841/j.issn.1007-4333.2014.02.15
投稿时间:2013-05-13
基金项目:农业废弃物高效循环利用关键技术研究(2012BAD14Ba);2008粤港关键领域重点突破项目(2009205200031);广东省2008年省部产学研结合项目(2008A090400001);农业部公益性行业科研专项(200803033)Chinese University Scientific Fund (2009TD15);吉林省科技发展计划项目(20115030)
Dynamic change of nitrifying bacteria in chicken manure sawdust aerobic composting process
CHEN Ya-juan, HUO Pei-shu, HAN Hui, LI Ji
(College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China)
Abstract:
High-temperature aerobic composting was conducted with chicken manure and sawdust as materials.The aim was to study the effect of different initial moistures and composting on ammonium nitrogen and nitrifying bacteria dynamic changes.The results showed that nitrifying bacteria existed among the entire composting process.The community structure of nitrifying bacteria greatly changed at the compost maturity stage.The concentration of ammonium nitrogen was related to nitrifying bacteria species richness and their stability.Ammonium nitrogen might induce in the growth of nitrifying bacteria.The moisture content of the compost was the key factor leading the change of nitrifying bacteria community structure. The data of T-RF150,169 and 343 bp showed the strong adaptability,in which most belonged to the genus and unculturable bacteria.The T3 treatment was benefit to the growth and stability of nitrifying bacteria community.
Key words:  chicken manure  composting  NH4+-N  nitrifying bacteria