| 本文已被：浏览 1137次 下载 589次 |
码上扫一扫！ |
| UV-A和UV-B提高甘蓝幼苗花青素含量以及调控基因表达分析 |
|
齐艳1, 邢燕霞1, 郑禾2, 孙倩倩1, 李殿波1, 王晋芳1, 石锦1, 赵冰1, 郭仰东1
|
|
|
| (1.中国农业大学 农学与生物技术学院, 北京 100193;2.北京市海淀区农业科学研究所, 北京 100080) |
|
| 摘要: |
| 为甘蓝作物中花青素的合成代谢研究提供理论基础，以“早红”（紫甘蓝）和“中甘11”（青甘蓝）为试验材料，利用UV-A和UV-B不同的时间组合照射生长4周的甘蓝幼苗，测定甘蓝中花青素的含量和代谢相关的主要的结构基因和转录因子在不同处理中的表达。试验表明：紫甘蓝和青甘蓝在经过UV-A和UV-B处理后，花青素含量均显著提高，并均在6 h达到最大值；UV-B处理比UV-A处理提高甘蓝花青素的含量方面更有效，在紫甘蓝和青甘蓝中分别提高41%和45%。分析花青素生物合成与代谢相关的结构基因和转录因子的表达情况可知UV-A和UV-B促进花青素积累的调节机制不同，甘蓝中不同品种积累花青素的机制也不同，综合分析结果显示，DFR和LDOX这2个下游结构基因在UV-A和UV-B不同处理以及紫甘蓝和青甘蓝不同品种间表达量都提高了，说明紫外照射提高花青素生物合成的下游结构基因的表达与甘蓝花青素的含量提高具有非常密切的关系。 |
| 关键词: 甘蓝 花青素 UV-A UV-B 结构基因 转录因子 |
| DOI：10.11841/j.issn.1007-4333.2014.02.13 |
| 投稿时间：2013-07-29 |
| 基金项目:国家重点基础研究发展计划（2012CB113900）；北京市现代农业产业体系（BLVT-03） |
|
| UV-A and UV-B involved in induction and regulation of anthocyanin biosynthesis in cabbage |
|
QI Yan1, XING Yan-xia1, ZHENG He2, SUN Qian-qian1, LI Dian-bo1, WANG Jin-fang1, SHI Jin1, ZHAO Bing1, GUO Yang-dong1
|
| (1.College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China;2.Agricultural Institute of Haidian, Beijing 100080, China) |
| Abstract: |
| This experiment was aimed at testing accumulations of anthocyanin in the cabbage seedlings and expression of the anthocyanin biosynthetic and regulatory genes under the environmental stimulus of UV-A and UV-B with "Zao Hong" (red cabbage) and "Zhong Gan 11" (green cabbage).The results showed that anthocyanin contents increased up to a peak after 6hs following UV-A and UV-B irradiation treatment in red and green cabbages.Compared to the UV-A treatment,the UV-B treatments was more effective in increasing anthocyanin accumulation.The analysis of the expressions of anthocyanin biosynthetic and regulatory genes showed that the regulatory mechanisms of UV-A and UV-B were different while concerning anthocyanin production and the accumulation mechanisms of red and green cabbages.Moreover,the downstream structure genes of DFR and LDOX were all up-regulated in the treatments.It was evident that the up-regulated expression of the downstream structure genes was closely related to the increase of anthocyanin content during UV-A and UV-B treatments. |
| Key words: cabbage anthocyanin UV-A UV-B structural gene transcription factor |
