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| 纤维素分解菌系WSD-5常温产酶高温糖化小麦秸秆研究 |
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温博婷1, 袁旭峰1, 华彬彬1, 尹永焕1, 王小芬1, 钟萼蓉2, 崔宗均1
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| (1.中国农业大学 农学与生物技术学院/中国农业大学生物质工程中心, 北京 100193;2.黑龙江省农垦科学院 经济作物开发研究所, 黑龙江 佳木斯 154007) |
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| 摘要: |
| 将木质纤维素原料转化为燃料乙醇，受到全世界的期待。本研究探讨了利用复合菌系直接分解糖化的新途径。结果显示，该复合菌系优化的产酶活条件是转速为0 r/min，底物添加量为3%，第6天酶活达到峰值，主要酶活为木聚糖酶活、CMC酶活和滤纸酶活，分别为15.18、3.43和0.79 IU/mL。在第15天培养结束后，WSD-5对小麦秸秆中纤维素、半纤维素以及木质素的分解率分别达到71.3%、90.6%和51.7%。在酶活高峰期，在添加底物的同时升温至55 ℃保温糖化，结果发现还原糖的浓度显著提高。酶解小麦秸杆产生的总还原糖达到125 mg/g以上，两次糖化总还原糖浓度为常温发酵时的15倍以上。当底物浓度为6%时的转化率最高，达到150.73 mg/g。在糖化的过程中，产生了少量的甲酸、乙酸等可溶性小分子化合物，可以作为甲烷发酵的底物，具有一定的利用意义。试验扩大到3 m3规模的中试试验。本研究为木质纤维素的低成本分解糖化提供了新的思路和途径。 |
| 关键词: 木质纤维素 复合菌系 复合酶系 糖化 |
| DOI：10.11841/j.issn.1007-4333.2014.02.06 |
| 投稿时间：2013-07-22 |
| 基金项目:国家公益性行业（农业）科研专项（201303080）；国家科技支撑计划项目（2012BAD14B01）；中国农业大学研究生科研创新专项（2012YJ015） |
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| Enzymatic digestibility by the composite microbial system of WSD-5：Enzyme production at room temperature and saccharification at high temperature |
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WEN Bo-ting1, YUAN Xu-feng1, HUA Bin-bin1, YIN Yong-huan1, WANG Xiao-fen1, ZHONG E-rong2, CUI Zong-jun1
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| (1.College of Agronomy and Biotechnology/Center of Biomass Engineering, China Agricultural University, Beijing 100193, China;2.Insititute of Crop Development, Heilongjiang Academy of Land-reclaimation Sciences, Jiamusi 154007, China) |
| Abstract: |
| The world is looking forward to the convertion of lignocellulose into bioethanol efficiently.This study applied a new approach to utilize composite microbial system for saccharification directly.The experiment expanded in the pilot scale of 3 m3.Experiment results showed that the highest enzyme activities were obtained when stirring rate was 0 rpm,and substrate loading rate was 3%.The highest cellulase and hemicellulase were obtained af day 6 after cultivation.The key enzyme activities in the fermentation broth were detected,CMCase was 3.43 IU/ml and Xylanase was 15.18 IU/ml.The weight loss of cellulose,hemicellulose and lignin were 71.3%,90.6% and 51.7%,respectively.After 6 days of fermentation,the crude enzyme were obtained and used for saccharification directly.The results showed that the concentration of reducing sugar significantly increased to 125 mg/g,which was more than 15 times than that in the fermentation broth.It peaked to 150.73 mg/g when the substrate loading rate was 6%.A small amount of organic acid was produced during saccharification,which were also the soluble small molecule compounds which would be used for biogas.This study provided a new idea and method of a low cost saccharification. |
| Key words: lignocellulose composite microbial system cellulolytic enzyme system saccharification |
