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| 植物乳杆菌F的筛选及对大鼠体脂的影响 |
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辛金鸽1, 倪学勤1,2, 曾东1,2, 王鹤松1, 李涛1, 沈雪娇1
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| (1.四川农业大学 动物医学院/动物微生态研究中心, 四川 雅安 625014;2.动物疫病与人类健康四川省重点实验室, 四川 雅安 625014) |
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| 摘要: |
| 为分离具有降胆固醇功能的菌株，并研究其对大鼠脂肪沉积的影响，通过模拟胃肠道环境以及降胆固醇试验，分离得到一株植物乳杆菌F（Lactobacillus plantarum F），在培养液中的胆固醇脱除率为55.03%。用不同浓度的该菌液饲喂SD大鼠（（130±2）g）8周（FⅠ组、FⅡ组和FⅢ组：分别灌胃2×106、2×108和2×1010 CFU/mL菌液）并饲喂高脂饲料。结果表明：试验Ⅰ、Ⅱ组体脂指数（腹部脂肪、肾周脂肪垫和附睾脂肪垫）、体重、肝脏体指数、甘油三酯（TG）、总胆固醇（TC）、谷丙转氨酶（ALT）和低密度脂蛋白胆固醇（LDL-C）相比高脂组均显著降低（P<0.05或P<0.01）；同时，高密度脂蛋白胆固醇（HDL-C）均增加，且试验Ⅰ组变化极显著（P<0.01）。另外，试验组盲肠内乳酸菌和双歧杆菌属数量均显著升高（P<0.05或P<0.01）。结果显示，植物乳杆菌F对于高脂饲料引起的大鼠肥胖具有明显的抑制作用，并与肠道菌群的改变有一定关系。 |
| 关键词: 植物乳杆菌F 降胆固醇 脂肪沉积 抑制作用 |
| DOI：10.11841/j.issn.1007-4333.2014.01.19 |
| 投稿时间：2013-06-02 |
| 基金项目:教育部留学回国人员科研启动基金资助项目（200835-1 ）；四川省学术带头人培养基金项目 |
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| Screening of the hypocholesterolemic effect and body fat control ability of Lactobacillus plantarum F |
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XIN Jin-ge1, NI Xue-qin1,2, ZENG Dong1,2, WANG He-song1, LI Tao1, SHEN Xue-jiao1
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| (1.College of Veterinary Medicine/Animal Microecology Institute, Sichuan Agricultural University, Ya'an 625014, China;2.Key Laboratory of Animal Disease and Human Health of Sichuan Province, Ya'an 625014, China) |
| Abstract: |
| The strain F with hypocholesterolemic activity was screened by imitating the gut conditions and reducing the cholesterol level in vitro,and its ability of reducing body fat accumulation in mice was investigated.Strain F was identified as Lactobacillus plantarum and could reduce 55.03% of cholesterol in the medium.Male SD rats with an average weight of (130±2)g were administered with different concentrations of strain F at 2×106,2×108 or 2×1010 CFU/mL (groupⅠ,Ⅱ and Ⅲ) for 8 weeks respectively,and were fed with high-fat feed.Compared with the high-fat group,the adipose tissue mass (abdominal fat,perirenal fat pad and epididymal fat pad),body weight,growth indexes of liver,TG,TC,ALT and LDL-C in groupⅠand Ⅱsignificantly reduced (P<0.05 or P<0.01),meanwhile,HDL-C was improved in the three F groups,especially in group Ⅰ(P<0.01).In addition,the number of Lactobacillus and Bifidobacterium significantly increased in the three F groups (P<0.05 or P<0.01).L.plantarum F with cholesterol-reducing function could reduce fat accumulation and enrich gut microbiota. |
| Key words: L. plantarum F cholesterol-reducing fatty deposition depressant effect |
