| 摘要: |
| 为解析月季失水诱导SNAC类转录因子RhNAC4的转录调控特性，利用染色体步移的方法分离了RhNAC4的启动子，并构建含GUS报告基因的载体转化拟南芥，分析了RhNAC4的启动子表达特性。结果表明：1）在月季中分离得到RhNAC4基因5’端上游1 753 bp的启动子序列；2）RhNAC4启动子序列中具有与发育、激素响应、非生物胁迫和生物胁迫等相关的顺式作用元件；3）获得了RhNAC4启动子驱动GUS基因的转基因拟南芥植株，GUS染色表明RhNAC4基因在植株不同的生长发育阶段和花器官中都有表达；4）失水胁迫、赤霉素、1-氨基环丙烷1-羧酸和甘露醇处理能够诱导RhNAC4的启动子活性，而盐和脱落酸处理对启动子活性的影响不显著。总之，月季切花失水胁迫诱导的SNAC类转录因子RhNAC4基因启动子含有逆境相关调控元件，其启动子在植株不同发育阶段和失水、乙烯等条件下具有转录活性。研究结果有助于解析RhNAC4参与月季切花失水胁迫耐性的作用机理。 |
| 关键词: 月季切花 RhNAC4启动子 顺式作用元件 转录活性 |
| DOI：10.11841/j.issn.1007-4333.2014.01.08 |
| 投稿时间：2013-05-03 |
| 基金项目:国家自然科学基金项目（31171992）；北京市科技新星计划（2009B51） |
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| Expression patterns of the promoter of rose RhNAC4 gene in Arabidopsis |
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ZHANG Xiao, JIANG Gui-mei, JIANG Xin-qiang, GAO Jun-ping, ZHANG Chang-qing
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| (College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China) |
| Abstract: |
| This study was aimed to investigate the transcriptional regulation of RhNAC4,a SNAC transcription factor gene induced by dehydration in rose petals (Rosa hybrida L.‘Samantha’).The promoter sequence of RhNAC4 was isolated by genomic walking.The transcription activities of RhNAC4 promoter were investigated in ProRhNAC4::GUS transgenic Arabidopsis.The results indicated that:the 1 753 bp promoter sequence of RhNAC4 was isolated from rose.The promoter contained different regulatory cis-acting elements,including development-,hormone-and abiotic or biotic stress-related cis-elements.We generated ProRhNAC4::GUS transgenic Arabidopsis.and the histochemical GUS staining revealed that the RhNAC4 promoter had transcription activities at the development stages of seedlings or floral organs in transgenic Arabidopsis.The transcription activities of RhNAC4 promoter were induced under different treatments,including dehydration,GA3,ACC and mannitol.It was evident that the RhNAC4 promoter contained stress-related cis-elements and had transcription activities during the developmental stages and under dehydration,ethylene etc.These results could contribute to the understanding the role of RhNAC4 involved in the dehydration tolerance of rose petals. |
| Key words: cut rose RhNAC4 promoter cis-element transcription activity |
