| 摘要: |
| 为研究甜瓜属种间杂交质体DNA的遗传规律,利用PCR直接测序法对甜瓜属异缘四倍体新种(Cucumis×hytivus Chen and Kirkbride.)S5自交后代及其杂交母本甜瓜属野生种(Cucumis hystrix Chakr.)和父本栽培黄瓜'北京截头’(Cucumis sativus cv. 'Beijingjietou’)叶绿体基因Matk-trnK和rbcL-accD区域的部分DNA序列进行比较分析。结果显示在长度为2 036 bp Matk-trnK区域中存在着18个多态性位点,其中有16个多态性位点的碱基子代与母本相同,只有2个位点与父本相同;在长度为945 bp的rbcL-accD区域中存在着19个多态性位点,其中有18个多态性位点的碱基子代与母本相同,只有1个多态性位点与父本相同。这表明甜瓜属hystrix×sativus种间杂交中质体DNA遗传主要表现为母系遗传。 |
| 关键词: 甜瓜 质体 多态性位点 母系遗传 |
| DOI：10.11841/j.issn.1007-4333.2013.06.020 |
| 投稿时间：2013-04-07 |
| 基金项目:国家自然科学基金重点项目(30830079); 教育部'111’项目(B08025); 江苏省三项工程项目(SXGC(2012)373); 江苏省青蓝工程项目资助 |
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| Polymorphic inheritance analysis of plastid DNA ininterspecific crossing of Genus Cucumis |
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WEI Yue1,2, YAN Zhi-ming2, WANG Quan-zhi2, BO Kai-liang1, WU Zhi-ming1, CHEN Jing-feng1
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| (1.Key Laboratory of Southern Vegetable Crop Genetics Improvement, Nanjing Agricultural University, Nanjing 210095, China;2.Polytechnical College of Agriculture and Forestry, Zhenjiang 212400, China) |
| Abstract: |
| To investigate the inheritance of plastid DNA in the interspecific crossing of Genus Cucumis,the DNA sequences in partial region of chloroplast Matk-trnK and rbcL-accD gene from cucumis allotetraploid(Cucumis×hytivus Chen and Kirkbride.)S5 selfing progeny,cultivated cucumber 'BeijingJietou’(C.sativus L.) the paternal parents and the wild Cucumis species(C.hystrix Chakr.) the maternal parents,were comparatively analysed,using PCR sequencing method.Eighteen polymorphic loci were detected in 2 036 bp length Matk-trnK gene region,sixteen polymorphic loci in allotetroploid as the its maternal parents,two as its paternal parents.Nineteen polymorphic loci were detected in 945 bp length rbcL-accD gene region,eighteen polymorphic loci in allotetraploid as its maternal parents,only one as its paternal parents.The result demonstrated that plastid genomes was maternal inheritance pattern in hystrix×sativusin species crosssing among Genus Cucumis. |
| Key words: Cucumis plastid polymorphic loci maternal inheritance |
