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| 温度及碳源对纤维素分解菌群分解活性与稳定性的影响 |
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吕育财1,2, 李宁3, 罗彬1, 龚大春1, 王小芬4, 崔宗均4
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| (1.三峡大学 化学与生命科学学院/新能源研究院, 湖北 宜昌 443002;2.车用生物燃料技术国家重点实验室, 河南 南阳 473000;3.三峡大学 水利与环境学院, 湖北 宜昌 443002;4.中国农业大学 农学与生物技术学院/生物质工程中心, 北京 100193) |
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| 摘要: |
| 为探索秸秆纤维素分解菌群筛选过程中,温度及碱处理小麦秸秆对菌群纤维素分解活性及菌群结构的影响,利用高温秸秆堆肥为筛选菌源,以碱处理小麦秸秆和未经碱处理小麦秸秆为碳源,分别在50和60℃条件下进行限制性筛选,最终获得18组具有纤维素分解活性的菌群。选择其中4组代表性菌群进行连续继代培养,监测相关性质,并利用PCR-DGGE技术结合主成分分析(PCA)方法对菌群结构进行分析。结果表明,从高温堆肥环境筛选秸秆纤维素分解菌群,培养温度及秸秆碳源均影响菌群的筛选效果。以碱处理小麦秸秆为碳源的菌群在分解秸秆过程中能够保持较好的菌群结构稳定性;60℃的温度条件和碱处理小麦秸秆的碳源条件更有利于获得高活性的纤维素分解菌群,并在此条件下成功筛选到菌群WDC2。该菌群分解碱处理小麦秸秆的纤维素内切酶活性(CMCase)达到1.01 U/mL,分解率最高为60.8%。 |
| 关键词: 小麦秸秆 纤维素分解 生物质能源 纤维素分解菌群 菌群结构 |
| DOI：10.11841/j.issn.1007-4333.2013.06.005 |
| 投稿时间：2013-05-08 |
| 基金项目:三峡大学人才启动基金(0620090045); "十二五"农村领域国家科技计划课题(2011BAD15B01); 车用生物燃料技术国家重点实验室开放基金资助项目(2013022) |
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| Effect of enriching condition with temperature and carbon onthe activity and stability of cellulose-degrading community |
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LV Yu-cai1,2, LI Ning3, LUO Bin1, GONG Da-chun1, WANG Xiao-fen4, CUI Zong-jun4
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| (1.College of Chemistry and Life Science/Research Institute for New Energy, China Three Gorges University, Yichang 443002, China;2.State Key Laboratory of Motor Vehicle Biofuel Technolog, Nanyang 473000, China;3.College of Hydraulic and Environmental Engineering, China Three Gorges University, Yichang 443002, China;4.College of Agronomy and Biotechnology/Center of Biomass Engineering, China Agricultural University, Beijing 100193, China) |
| Abstract: |
| This study aims to explore the effect of temperature and carbon on cellulose-degrading activity and structure of community,and 18 groups of cellulose-degradation community were enriched from high-temperature composting of straw.There were two kinds of wheat straw:wheat straw with alkali treatment and wheat straw without alkali treatment using as a carbon source,which were cultured at 50 and 60℃ respectively.4 typical groups from the 18 communities were continuously transferred.Some characteristics with the community were detected,and the structure of communities was analyzed by PCR-DGGE technique combined with a principal component analysis(PCA).The results showed that the incubation temperature and straw materials affected the enriched effect of straw-degrading community from the thermophilic composting environment.The communities with alkali treatment of wheat straw as a carbon source may keep the stability of community structure in process of straw-degradation.The culture conditions with the temperature of 60℃ and wheat straw with alkali treatment as a carbon source were conducive to obtaine a high activity of the cellulose degrading community.A community WDC2 was successfully enriched with the above conditions.The cellulose endonuclease activity(CMCase) reached 1.01 U/mL,and the degradation rate was up to 60.8 % in the process of alkali treatment of wheat straw,which was degraded by community WDC2. |
| Key words: wheat straw cellulose-degradation bioenergy cellulose-degrading community community structure |
