| 本文已被：浏览 1551次 下载 841次 |
码上扫一扫！ |
| 利用体外产气法研究植物精油对瘤胃体外发酵和甲烷生成的影响 |
|
金恩望1,2, 王加启1, 卜登攀1, 周凌云1, 孙鹏1, 姜雅慧1, 包万华1, 史浩亭1,2, 李发弟2
|
|
|
| (1.中国农业科学院 北京畜牧兽医研究所/动物营养学国家重点实验室, 北京 100193;2.甘肃农业大学 动物科学技术学院, 兰州 730070) |
|
| 摘要: |
| 为探讨植物精油对瘤胃体外发酵的影响,采用体外产气法分析不同添加水平的茶树油、肉桂油和丁香油对瘤胃发酵模式、发酵产气量及动力学参数和瘤胃氮代谢的影响。与对照组相比结果表明:1)添加各剂量的茶树油和肉桂油对微生物蛋白(MCP)含量无显著影响,但氨氮(NH3-N)浓度均有降低趋势,其中添加300和1 500 mg/L肉桂油显著降低了NH3-N浓度(P<0.05)。2)添加1 000 mg/L的茶树油或1 500 mg/L的肉桂油显著降低了总挥发性脂肪酸(TVFA)和丙酸浓度(P<0.05),而50和100 mg/L茶树油处理组丁酸浓度显著升高(P<0.05),300和500 mg/L的肉桂油处理组支链脂肪酸浓度显著升高(P<0.05)。3)添加100 mg/L的茶树油和丁香油均可显著提高理论最大发酵产气量(b),并且所有植物精油添加组甲烷(CH4)体积分数均有降低。添加100 mg/L的茶树油或100和300 mg/L的肉桂油有助于改善瘤胃微生物发酵。 |
| 关键词: 植物精油 体外产气法 瘤胃体外发酵 CH4 |
| DOI：10.11841/j.issn.1007-4333.2013.03.018 |
| 投稿时间：2012-09-10 |
| 基金项目:"十二五"国家科技支撑计划课题(2012BAD12B02-5); 中国科学院知识创新工程重要方向项目(XDA01010301) |
|
| Effects of essential oil on rumen fermentation and methanogenesis in vitro gas production |
|
JIN En-wang1,2, WANG Jia-qi1, BU Deng-pan1, ZHOU Ling-yun1, SUN Peng1, JIANG Ya-hui1, BAO Wan-hua1, SHI Hao-ting1,2, LI Fa-di2
|
| (1.State Key Laboratory of Animal Nutrition/Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100193, China;2.Collage of Animal Science and Technology Gansu Agricultural University, Lanzhou 730070, China) |
| Abstract: |
| An in vitro cumulative gas production trial was conducted to study the effects of tea tree oil,cinnamon oil,and clove bud oil on subsequent 72 h rumen fermentation and methanogenesis at different dose.The results showed that,1) there were no effect on the production of microbial crude protein,but the concentration of ammonia N were decreased with added tea tree oil and cinnamon oil,especially when the cinnamon oil at the dose of 300 or 1 500 mg/L(P<0.05).2) the production of total volatile fatty acid (TVFA) and concentration of propionic acid were decreased by adding 1 000 mg/L tea tree oil or 1500 mg/L cinnamon oil.At dose of 50 and 100 mg/L,the tea tree oil increased the concentration of butyrate acid.The production of iso-butyrate and iso-valerate acid were increasing with 300 and 500 mg/L cinnamon oil.3) At 100 mg/L,tea tree oil and clove bud oil were significant different on the theory maximum gas production compared with the control groups.All treatments tended to decrease the methane concentration (P<0.05).In general,the tea tree oil at the level of 100 mg/L and cinnamon oil at the 100 and 300 mg/L may be allowed to manipulation of rumen microbial fermentation. |
| Key words: essential oil in vitro gas production rumen fermentation in vitro methane |
