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| 酶解蚕豆蛋白制备多肽的工艺优化及多肽酒的发酵 |
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杨希娟1,2, 党斌1,2, 耿贵工1,2, 刘玉皎2,3
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| (1.青海省农林科学院 青藏高原农产品加工重点实验室,西宁 810016;2.青海省高原作物种质资源创新与利用-国家重点实验室培育基地,西宁 810016;3.青海大学 农林科学院,西宁 810016) |
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| 摘要: |
| 以蚕豆蛋白为原料,采用碱性蛋白酶酶解、酒精发酵制备蚕豆多肽酒,对其加工工艺进行了研究。结果表明:1)蚕豆蛋白酶解的优化工艺为:蚕豆蛋白质量浓度32 g/L,水解温度43.2 ℃,酶用量9 821.12 U/g,pH9.5,在此条件下酶解2 h,蚕豆蛋白的水解度达到19.64%;2)以蚕豆酶解液为原料制备多肽酒的发酵工艺为:加糖量200 g/L,酵母接种量0.22%,发酵温度28 ℃,发酵时间6 d,在此条件下制得的蚕豆多肽酒的酒精体积分数为9.6%;发酵结束后添加柠檬酸1.5 g/L,白砂糖70 g/L,环糊精6 g/L时,产品风味较好,显著降低了产品的苦味。 |
| 关键词: 蚕豆 蛋白 酶解 发酵 多肽酒 |
| DOI：10.11841/j.issn.1007-4333.2013.02.025 |
| 投稿时间：2012-06-04 |
| 基金项目:农业科技成果转化资金项目(2011GB2G200002); 现代农业产业技术体系建设专项(CARS-09); 青海高校"135高层次人才培养工程"专项 |
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| Optimization of preparation technology for broad bean polypeptide and fermentation of polypeptide wine |
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YANG Xi-juan1,2, DANG Bin1,2, GENG Gui-gong1,2, LIU Yu-jiao2,3
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| (1.Tibetan Plateau Laboratory of Agric-Product Processing,Qinghai Academy of Agriculture and Forestry,Xining 810016,China;2.The Cultivating Base of National Key Lab-Plateau Crop Gene Innovated and Utilized in Qinghai Province,Xining 810016,China;3.Academy of Agriculture and Forestry,Qinghai University,Xining 810016,China) |
| Abstract: |
| In order to obtain the broad bean peptides wine,broad bean protein was hydrolyzed by alkaline protease and then was alcoholically fermented in this experiment.The preparation technology of broad bean peptides wine was investigated.The result showed that the optimum conditions for the protein hydrolysates of broad bean were broad bean protein concentration 32 g/L,enzymatic hydrolysis temperature 43.2 ℃,dosage of enzyme 9 821.12 U/g and pH value 9.50,under which the degree of hydrolysis was 19.64%.The fermentation condition of broad bean peptides wine was 200 g/L sugar,0.22% yeast quantity,and 28 ℃ for 6 days.The alcohol content of broad bean peptides wine is 9.6%.In order to mitigate the bitterness of the original wine,1.5 g/L Citric acid,70 g/L sugar and 6 g/L β-CD were added and the final product had a good mouth feel. |
| Key words: broad bean protein hydrolysis fermentation peptides wine |
