| 摘要: |
| 为研究日粮添加共轭亚油酸(Conjugated linoleic acid,CLA)对细胞免疫抑制剂环孢酶素A(Ciclosporin A,CsA)注射仔猪细胞免疫反应的影响,采用2×2试验设计即日粮类型(2%CLA或2%大豆油)和细胞免疫抑制处理(注射CsA或生理盐水),48头21 d断奶仔猪随机分为4个处理组,试验期为42 d。注射CsA显著提高仔猪的料重比(P<0.05),而日粮添加CLA显著降低仔猪的料重比(P<0.05),且日粮CLA阻止因CsA注射引起的仔猪料重比提高(P<0.05)。CsA注射极显著降低胸腺CD4+ 和CD8+T淋巴细胞百分数(P<0.01)。日粮CLA显著提高胸腺CD4+ CD8+双阳性和CD8+单阳性T淋巴细胞百分数(P<0.05),且阻止注射CsA引起的仔猪胸腺CD4+单阳性、CD8+单阳性T淋巴细胞百分数的降低(P<0.01)。CsA注射严重耗竭仔猪外周血CD3+、CD4+ 和CD8+T淋巴细胞(P<0.01)。无论注射CsA与否,日粮添加CLA均提高仔猪外周血CD8+T淋巴细胞数和IL-2诱生活性(P<0.05),显著缓解注射CsA引起仔猪外周血CD3+、CD4+ 和CD8+T淋巴细胞数的减少和IL-2诱生活性的降低(P<0.01)。在无CsA注射的情况下,日粮CLA对仔猪外周血淋巴细胞转化率没有显著影响(P>0.05),但日粮CLA显著抑制因CsA注射引起的仔猪外周血淋巴细胞转化率降低(P<0.05)。日粮添加CLA能显著缓解因CsA注射引起的仔猪细胞免疫抑制。 |
| 关键词: 仔猪 共轭亚油酸 环孢酶素A 细胞免疫反应 |
| DOI：10.11841/j.issn.1007-4333.2013.02.020 |
| 投稿时间：2012-06-05 |
| 基金项目:河南省科技厅重点科技公关资助项目(082102110123); 郑州牧专博士基金项目(2007DSR003) |
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| Effects of dietary conjugated linoleic acid on cell immunity response of piglets after CsA injection |
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LIU Yong-xiang1,2, CHENG Wei1,2, QIU Rong-chao2, JIANG Dong-feng2
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| (1.Henan Engineering Technology Research Center of Animal Nutrition and Feed,Zhengzhou 450011,China;2.Zhengzhou College of Animal Husbandry Engineering,Zhengzhou 450011,China) |
| Abstract: |
| The present experiment was conducted to investigate effects of conjugated linoleic acid (CLA) on cell immunity response of piglets after Cyclosporin A (CsA) injection.The experiment design was a 2×2 factrial design,and the main factors consisted of diets (2% CLA or 2% soybean oil)and immunosuppression treatment(CsA or saline injection).Fourty-eight weaned piglets at 21days were randomly allocated into four experimental groups.The experimental period was 42 days.CsA injection significantly increased Feed/Gain (F∶G) of piglets(P<0.05),however dietary CLA significantly decreased F∶G of piglets(P<0.05),moreover CLA diets ameliorated the increase of F∶G resulted from CsA injection (P<0.01).CsA-treatment significantly decreased the percentage of CD4+ and CD8+T lymphocytes in thymus (P<0.01).2% CLA diets resulted in higher percentage of CD4+ CD8+ doublepositive and CD8+ siglepositive T lymphocytes in thymus(P<0.05),and decrement of CD4+ and CD8+ T lymphocytes of thymus resulted from CsA injection was lesser in piglets fed the CLA diet compared with those fed basal diets(P<0.01).CsA-treatment significantly depleted the number of CD3+, CD4+ and CD8+T lymphocytes of peripheral blood.Whether with CsA injection or not,dietary CLA significantly increased CD8+ T lymphocyte subpopulations of peripheral blood and IL-2 production(P<0.05).The decrement of CD3+ ,CD4+ and CD8+ T lymphocyte of peripheral blood and IL-2 production was notably suppressed in piglets fed the CLA diet(P<0.01).Without CsA injection treatment,dietary CLA didn't influence lymphocyte proliferation of peripheral blood(P>0.05),but notably suppressed the decrease of lymphocyte proliferation resulted from CsA injection(P<0.05).Dietary CLA effectively ameliorated cell immunosuppression resulted from CsA injection. |
| Key words: piglets CLA CsA cell immune response |
