| 摘要: |
| 为评价大叶草樱对根癌病的抗性,以4个月的实生大叶草樱为材料,采用人工接种根癌土壤杆菌(Agrobacterium tumefaciens Conn)和发根土壤杆菌(A. rhizogenes Conn)的方法研究了大叶草樱对根癌病的抗性及其抗性分离情况。结果表明:60 d后,大叶草樱接种根癌土壤杆菌的最大瘤径为8.3 mm,病情指数为23.65,为中度抗病类型(MR);对发根土壤杆菌的最大瘤径为4.7 mm,病情指数为18.63,为高度抗病类型(HR)。大叶草樱群体内对根癌土壤杆菌和发根土壤杆菌的抗性均存在显著的分离现象,其实生群体对根癌土壤杆菌存在免疫、高度抗病、中度抗病和低度抗病和感病5种抗病类型,分别占32.69%、31.73%、24.04%、7.69%和3.85%;对发根土壤杆菌存在免疫、高度抗病和中度抗病3种抗病类型,分别占16.67 %、73.53%和9.80%。大叶草樱是优异的抗根癌病种质资源。 |
| 关键词: 大叶草樱 根癌病 根癌土壤杆菌 发根土壤杆菌 抗性评价 |
| DOI：10.11841/j.issn.1007-4333.2013.02.014 |
| 投稿时间：2012-03-08 |
| 基金项目:国家"948"项目(201035) |
|
| Resistance evaluation of Dayecaoying to crown gall disease |
|
SHAO Shan-shan, QU Yan-hua, CHAI Peng, ZHU Li-xin, JIA Ke-gong
|
| (College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,China) |
| Abstract: |
| In order to evaluate the resistance of Dayecaoying to crown gall disease,pot seedlings were used as test materials via artificial inoculation,mainly to A.tumefaciens Conn and A.rhizogenes Conn.The results showed that Dayecaoying was excellent germplasm resource resisting to crown gall disease.At 60 days after inoculation,the disease index of Dayecaoying to A.tumefaciens Conn was 23.65,while it was 18.63 to A.rhizogenes Conn.According to the resistance evaluation standard to Crown gall disease,it moderately resisted to A.tumefaciens Conn and was separated into 5 types of disease resistance,including being immune,highly resistant,moderately resistant,low resistant and susceptible with corresponding percentages of 32.69%,31.73%,24.04%,7.69% and 3.85% respectively.And it was highly resisted to A.rhizogenes Conn and was divided into 3 types of disease resistance according to the evaluation standard with corresponding percentages of 16.67 %,73.53% and 9.80% respectively. |
| Key words: Dayecaoying crown gall disease Agrobacterium tumefaciens Conn A. rhizogenes Conn resistance evaluation |
