| 摘要: |
| 为寻找小桐子枝叶中的杀虫活性物质,以具有杀虫作用的小桐子枝叶乙醇粗提物的石油醚萃取相为试验材料,对其采用硅胶柱反复柱层析,结合薄层层析制备分离及对各阶段分离产物进行活性追踪筛选,获得具有杀虫作用的活性部位AJ1,初步鉴定出活性部位主体成分。结果表明:AJ1活性组分在质量浓度为8 mg/mL时,对桃蚜(Myzus persicae(Sulzer))和菜青虫(Pieris rapae L.)的24 h(72 h)校正死亡率均大于70%,与吡虫啉无显著差异,显示出极高的毒杀活性。对AJ1组分进行GC-MS分析,初步鉴定了含量最高的9个化合物,为总量的96.18%,其中含量前5位的物质依次是1-烯丙氧基,2-异丙醇、4-乙炔基-1,2-二甲基-4喹啉醇、4-烯丙氧基-2-甲基-2-戊醇和2,4-二甲基癸烷和1-(已氧基)辛烷,5种主要成分为总量的83.81%。 |
| 关键词: 小桐子 石油醚萃取相 杀虫物质 生物活性 分离鉴定 |
| DOI：10.11841/j.issn.1007-4333.2013.01.023 |
| 投稿时间：2012-05-03 |
| 基金项目:云南省基金项目(2011FB086) |
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| Isolation and identification insecticidal activity franctions from petroleum ether partitioned extract in Jatropha curcas L. branches and leaves |
|
LI Yu-chuan, HUANG He-ping, YIN Li-xin, LI Shao-ping, WANG Chong-liang
|
| (School of Agriculture, Kunming University, Kunming 650214, China) |
| Abstract: |
| To make clear insecticidal activity components from Jatropha curcas L.branch leaves.Using alcohol crude extract of petroleum ether partitioned extract from Jatropha curcas L.branch leaves as an experimental material in our investigation,and then silica gel chromatography,combined with PTLC preparation.At same time,the each stage of separated products was carried out by tracking activity,analysis by GC-MS,structure elucidation the main compositions of exhibited active components.The results show that the concentration of colony AJ1 reached 8 mg/mL,Myzus persicae(Sulzer)and Pieris rapae L.both corrected mortality exceeded 70% in 24 h(72 h),showed no significant differences with imidacloprid(70%),showed that toxic activity was very high.The colony AJ1 analysis using GC-MS,the results showed,nine compounds with maximum contents were identification,total amount up to 96.18%,the first five of maximum contents were 2-Propanol,1-(allyloxy),4-Quinolnol,4-ethyldecahydro-1,2-dimethyl-,4-(allyloxy)-2-methyl-2-pentanol,2,4-Dimethyldecane and 1-(hexyloxy)octane.The total amount of five main constituents reached 83.81%. |
| Key words: Jatropha curcas petroleum ether partitioned extract insecticidal components biological activity isolation and identification |
