| 摘要: |
| 通过微列阵芯片(Microarray)分析,从珠眉海棠盐胁迫cDNA 文库中分离得到MzSTO(Salt tolerance protein)全长基因。结果表明:MzSTO 基因cDNA 全长1 066 bp,开放阅读框共729 bp,5'-UTR和3'-UTR 的长度分别是49 bp 和288 bp;MzSTO 编码242 个氨基酸,N 端有2 个保守的B-box 锌指结构域(CX2CX8CX7CX2CX4HX8H);半定量RT-PCR 表明MzSTO 基因受到盐和干旱胁迫抑制,受冷处理诱导,并响应ABA(abscisic acid)。以上结果表明MzSTO 基因可能通过依赖ABA 的途径参与非生物逆境胁迫。 |
| 关键词: 珠眉海棠 MzSTO 非生物胁迫 RT-PCR |
| DOI：10.11841/j.issn.1007-4333.2013.01.013 |
| 投稿时间：2012-04-17 |
| 基金项目:国家自然科学基金资助项目(31171940); 北京市自然科学基金资助项目(6112012) |
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| Cloning and expression analysis of salt stress MzSTO gene from Malus zumi Mats |
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JIANG Yu-zhuang, LI Ling-zi, ZHAO yu, LI Qing-tian, LIANG Zhan-feng, KONG Jin
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| (College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China) |
| Abstract: |
| The full-length cDNA sequence of MzSTO gene was isolated from a cDNA library of Malus zumi.The full-length cDNA of MzSTO was 1 066 bp.The 5'-UTR and the 3'-UTR were 49 and 288 bp respectively.Its open reading frame of 729 bp encoded a protein of 242 amino acids.There were two B-box zinc-finger domains (CX2CX8CX7CX2CX4HX8H) near N-terminal.MzSTO was induced by low temperature stress and repressed by high-salinity and drought treatment,which was,also responsive to ABA (abscisic acid) treatment by semi-quantative RT-PCR.All the results showed that MzSTO was involved in response of abiotic stresses including salt, drought and cold stresses through a possible ABA-dependent pathway in Malus zumi. |
| Key words: Malus zumi Mats MzSTO abiotic stresses RT-PCR |
