| 摘要: |
| 为确定秸秆可溶性糖测定时样品的最佳前处理条件,研究单因素对玉米秸秆和小麦秸秆可溶性糖得率的影响。利用中心组合试验(Box-Behnken)和响应曲面法(RSM)对主要前处理参数进行优化,并得到回归模型。结果表明:固液比(秸秆质量与水体积比)、水浴温度、提取时间和存放时间对玉米秸秆和小麦秸秆可溶性糖得率影响显著(P<0.05)。回归方程较好的反映了可溶性糖得率与固液比、水浴温度和提取时间的关系。玉米秸秆可溶性糖测定的最佳前处理条件为,固液比0.31 g/mL,水浴温度94℃,提取时间63 min,此条件下的可溶性糖得率为3.85%。小麦秸秆可溶性糖测定的最佳前处理条件为,固液比0.33 g/mL,水浴温度95℃,提取时间58 min,此条件下的可溶性糖得率为3.39%。 |
| 关键词: 玉米秸秆 小麦秸秆 可溶性糖 蒽酮硫酸法 前处理 响应曲面 |
| DOI：10.11841/j.issn.1007-4333.2012.05.016 |
| 投稿时间：2012-03-21 |
| 基金项目:公益性行业(农业)科研专项(201003063);土壤植物机器系统技术国家重点实验室开放课题基金(2011-SKL) |
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| Optimization of pretreatment for determination of soluble sugar in straw |
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NIU Wen-juan, XIAO Wei-hua, LIU Xian, FENG Lun, FAN Chong-xin, HAN Lu-jia
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| (College of Engineering, China Agricultural University, Beijing 100083, China) |
| Abstract: |
| In order to determine the optimal pretreatment condition of soluble sugar in corn stover and wheat straw,different factors were studied,Box-Behnken and RSM were used for the optimization of pretreatment and regression models were obtained.The results showed that the ratio of straw to water,water-bath temperature,extraction time and stored time had significant effects,P<0.05,on the yield of soluble sugar in corn stover and wheat straw.The optimal pretreatment condition of soluble sugar in corn stover is that the ratio of straw to water is 0.31 g/mL,water-bath temperature is 94℃,extraction time is 63 min,then the yield of soluble sugar in corn stover reach 3.85%.the optimal pretreatment condition of soluble sugar in wheat straw is that the ratio of straw to water is 0.33 g/mL,water-bath temperature is 95℃,extraction time is 58 min,then the yield of soluble sugar of wheat straw reach 3.39%. |
| Key words: corn stover wheat straw soluble sugar anthrone sulfuric acid method pretreatment RSM |
