| 摘要: |
| 通过不同浓度(0.001、0.010、0.100和1.000 μmol/L)冠菌素(COR)处理忍冬花和叶,应用高效液相色谱法(HPLC)测定其中有效成分含量变化。结果表明:不同浓度COR处理对忍冬花、老叶和新叶中主要成分含量产生影响,1) 0.010 μmol/L处理使忍冬花中的绿原酸和芦丁含量分别增加19.75%和13.80%,总体趋势表现为低浓度促进作用;2) 1.000 μmol/L处理使忍冬老叶中的绿原酸、咖啡酸、木犀草苷、异绿原酸A和异绿原酸C,分别较对照高49.72%、28.57%、56.64%、72.78%和49.08%,总体趋势表现为高浓度促进作用;3) 1.000 μmol/L处理使忍冬新叶中的异绿原酸A含量增加16.83%,对木犀草苷和总含量都有促进作用。由此可以看出,采用花可选用0.010 μmol/L冠菌素处理,采用叶可选用1.000 μmol/L冠菌素处理;这可为更好地开发利用忍冬提供理论依据。 |
| 关键词: 忍冬 高效液相色谱法 冠菌素 绿原酸 木犀草苷 |
| DOI：10.11841/j.issn.1007-4333.2012.05.010 |
| 投稿时间：2012-03-20 |
| 基金项目:国家科技重大专项(2009ZX09308-002);国家公益性行业(农业)科研专项(200903001-2-3) |
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| Influence of Coronatine treatments on the active ingredient contents of Lonicera japonica flowers and leaves |
|
NIU Xiao-xue, CHEN Xiao-wen, CUI Xu-sheng, SU He, GUO Yu-hai, DONG Xue-hui
|
| (College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China) |
| Abstract: |
| High performance liquid chromatographic (HPLC) method was applied to research the influence of Coronatine(COR) treatments of different concentrations on active ingredients of Lonicera japonica.The proper concentrations on different positions of Lonicera japonica have been confirmed which supply theoretical basis for exploitation.The results indicated that COR treatments of different concentrations had a certain impact on the main compound contents of flowers,old leaves and new leaves in Lonicera japonica:1) Concentration of 0.010 μmol/L has increased 19.75% and 13.80% respectively on the contents of chlorogenic acid and rutin in honeysuckle flower,and the overall trend was promoted at a low concentration;2) Concentration of 1.000 μmol/L has increased the contents of chlorogenic acid,coffeic acid,luteolin-7-o-glucoside,isochlorogenic acid A and C in old leaves with 49.72%,28.57%,56.64%,72.78% and 49.08% respectively,and the high concentration had a stimulation effect;3) Concentration of 1.000 μmol/L has increased 16.83% on the content of isochlorogenic acid A and has a stimulation effect on luteolin-7-o-glucoside and the total ingredients.It was concluded that COR treatments of 0.010 and 1.000 μmol/L can be applied to honeysuckle flowers and leaves of Lonicera japonica respectively. |
| Key words: Lonicera japonica high performance liquid chromatography (HPLC) Coronatine chlorogenic acid luteolin-7-o-glucoside |
