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| 利用体细胞核移植技术生产优秀种公猪 |
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薛振华1, 潘登科2, 朱振营3, 罗学明2, 云鹏1, 王晓凤1, 肖炜1
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| (1.北京市畜牧兽医总站, 北京 100107;2.中国农业科学院 北京畜牧兽医研究所, 北京 100193;3.中国检验检疫科学研究院 动植物检疫研究所, 北京 100029) |
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| 摘要: |
| 为探讨体细胞核移植技术用于种公猪的实际生产,复制优秀种公猪的可行性,精选优秀种公猪作为供体,取其耳组织成纤维细胞作核供体,体外成熟的猪卵母细胞为核受体构建克隆胚胎,胚胎体外培养后进行移植。试验先后移植了3 437枚1~4细胞期克隆胚胎到18头受体母猪的输卵管内,28 d 经B超检测,有16头受体母猪妊娠(88.89%),11头妊娠足月(68.75%)分娩产下53头仔猪,体细胞克隆猪的生产效率为2.80%(出生仔猪/移植胚胎)。早期生长性状测定结果显示,0 和7 d时克隆猪的体质量和体尺与普通公猪无显著差异(P>0.05)。利用体细胞核移植技术生产克隆种公猪,可以延伸种公猪的利用时间和空间,放大优秀种猪的利用价值。 |
| 关键词: 种公猪 体细胞核移植 胚胎移植 克隆 |
| DOI：10.11841/j.issn.1007-4333.2012.04.020 |
| 投稿时间：2012-02-27 |
| 基金项目:北京市农业科技项目(20090612);北京市优秀人才资助项目(2010D002006000004);生猪产业技术体系北京市创新团队 |
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| High performance boar cloned from somatic cell nuclear transfer |
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XUE Zhen-hua1, PAN Deng-ke2, ZHU Zhen-ying3, LUO Xue-ming2, YUN Peng1, WANG Xiao-feng1, XIAO Wei1
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| (1.Beijing Municipal Animal Husbandry and Veterinary General Station, Beijing 100107, China;2.Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;3.Institute of Animal and Plant Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100029, China) |
| Abstract: |
| To investigate the feasibility of the cloned high performance breeding boar by the technology of somatic cell nuclear transfer in actual production.The nuclear donors were selected from boars,ear organization cells,and the nuclear receptors come from some oocytes through in-vitro maturation.There are 3 437 pieces of 1~4 cells cloned embryos were transplanted to 18 receptors successively.Sixteen receptors were pregnancy (88.89%) at 28 d by B ultrasonic detection,and 53 piglets were obtained from 11 pregnancy receptors (68.75%).The cloned production efficiency was 2.80% (birth piglet/embryos).The determination results of growth characteristics showed that there is no significant difference in the measurement of cloned bodyweight and length (P>0.05) from ordinary boars at 0 and 7 d.Therefore,the cloned boars from the technology of somatic cell nuclear transfer can extend the space and span of a boar and enlarge the use value of the outstanding boar. |
| Key words: boar somatic cell nuclear transfer embryo transfer clone |
