| 摘要: |
| 利用Agilent棉花寡核苷酸表达谱芯片,以2个同核异质的红麻材料P3A和P3B为试材,分析了棉花表达谱芯片应用于红麻表达谱研究的可行性。结果表明:芯片杂交扫描图像信号清晰,信噪比较高,整体背景较低,各阳性质控点信号均匀一致,空白点和阴性质控点信号低。P3A在棉花的寡核苷酸芯片上共检测到37 640个表达位点,占芯片总位点的85.93%;P3B在棉花的寡核苷酸芯片上共检测到37 629个表达位点,占芯片总位点的85.90%。34 290个探针在不育系和保持系中均具有显著的杂交信号,占总探针数43 803的78.28%。表明红麻花药cRNA与棉花表达谱芯片上寡核苷酸探针之间的远缘杂交非常有效,用棉花寡核苷酸芯片研究红麻的基因表达是可行的。 |
| 关键词: 红麻 棉花 表达谱芯片 异源杂交 基因表达 |
| DOI：10.11841/j.issn.1007-4333.2012.04.003 |
| 投稿时间：2012-01-15 |
| 基金项目:国家自然科学基金资助项目(C130406);国家麻类产业技术体系岗位专家专项资金(nycytx-19) |
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| Feasibility of cotton oligo microarray for anther tissue gene expression study in kenaf (Hibiscus cannabinus L.) |
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JIN Gang1,2, TANG Xiang-min1, ZHOU Qiong1, CHEN Peng1, ZHOU Rui-yang1
|
| (1.College of Agriculture, Guangxi University, Nanning 530005, China;2.Guangxi Subtropical Crops Research Institute, Nanning 530001, China) |
| Abstract: |
| We explored tentatively expression patterns of two iso-nuclear alloplasmic lines in kenaf (P3A and P3B) by using a oligo microarray from cotton.The results showed that the signal of microarray hybridization was clear,and the images had a lower background and higher signal-noise ratio.Expression for 85.93% (37 640) of 43 803 probes was detected in anthers of P3A,and expression of 85.90% (37 629) of probes was observed in anthers of P3B.Of the probes on the arrays,34 290 (78.28%) consistently hybridized to labeled cotton probe in P3A and P3B.High heterologous hybridization rate suggest that it is viable using cotton oligo microarray to investigate gene expression in kenaf.We could successfully demonstrate that heterologous microarray hybridizations allow deep insights into the transcriptom of kenaf. |
| Key words: kenaf (Hibiscus cannabinus L.) cotton oligo microarray heterologous hybridization gene expression |
