| 摘要: |
| 为研究富含n-3或n-6 多不饱和脂肪酸(polyunsaturated fatty acid,PUFA)日粮对小鼠卵母细胞体外发育和线粒体功能的影响,把90只14 g左右昆明雌鼠随机分为3组,分别饲喂40 g/kg豆油日粮,40 g/kg鱼油日粮或40 g/kg共轭亚油酸(conjugated linoleic acid,CLA)日粮30 d;注射雌激素诱导小鼠超数排卵后,检测卵母细胞活性线粒体分布、线粒体钙浓度、膜电位以及脂肪滴分布;利用体外受精技术,评价卵母细胞体外发育能力。结果表明:相对于每个活性线粒体的分布,饲喂鱼油日粮的小鼠卵母细胞内层钙的分布比豆油日粮组有所升高(P <0.05),但是各组卵母细胞的活性线粒体比例和膜电位均没有显著性改变。另外,鱼油日粮组小鼠卵母细胞内脂滴密集,而CLA日粮组小鼠卵母细胞脂肪滴分布不均匀,每个卵母细胞所含脂肪较少。各日粮组卵母细胞受精率(2-细胞)没有显著性差异,但是CLA日粮组受精卵发育至桑葚胚和囊胚的比率分别比豆油组降低51.80%(P<0.05)和62.19%(P<0.05),比鱼油组降低52.97%(P<0.05)和60.89%(P<0.05)。因此,小鼠饲喂鱼油日粮可扰乱卵母细胞内钙平衡,但未改变其体外发育能力;CLA日粮可能通过促进卵母细胞内脂肪的降解从而降低受精卵体外发育率。 |
| 关键词: 鱼油 共轭亚油酸 小鼠 卵母细胞 线粒体 囊胚 |
| DOI：10.11841/j.issn.1007-4333.2012.03.017 |
| 投稿时间：2011-12-03 |
| 基金项目:国家杰出青年科学基金项目(30425037) |
|
| Effects of diet supplemented fish oil or conjugated linoleic acid (CLA) on mitochondrial function and developmental ability of the mice oocyte |
|
YI Dan1, ZENG Shen-ming2, GUO Yu-ming1
|
| (1.State Key Laboratory of Animal Nutrition/College of Animal Science and Technology,China Agricultural University,Beijing 100193,China;2.Laboratory of Animal Embryonic Biotechnology/College of Animal Science and Technology,China Agricultural University,Beijing 100193,China) |
| Abstract: |
| The study was conducted to investigate the effects of a diet supplemented n-3 or n-6 polyunsaturated fatty acid (PUFA) on mitochondrial function and developmental ability of mice oocyte.Ninety Kunming weaning mice with 14 grams body weight were randomly assigned to three groups,and feed with a diet of 4% soybean oil,4% fish oil,or 4% conjugated linoleic acid (CLA).After 30 days,the mice were treated with PMSG and hCG to induce superovulation.Oocytes were collected to examine mitochondrial parameters (active mitochondrial distribution,mitochondrial calcium,membrane potential and fat droplet distribution),and to assess embryo developmental ability after in vitro fertilization.The results showed that:1) Though there were no differences in active mitochondrial distribution,mitochondrial calcium,and membrane potential between different diets,the ratio of mitochondrial calcium to active mitochondria increased significantly in animals fed with a diet of fish oil (P<0.05);2) The distribution of fat droplets within oocytes of fish oil group were intensive,whereas less and non-uniform fat droplet were observed in oocytes of CLA group;3) Consumption of CLA significantly reduced the ability of the in vitro-fertilized zygotes to cleave and develop to either morula or blastocyst stage by 38.12% and 30.67% respectively,even though no difference was observed in in vitro fertility among these three diets.These results suggested that a diet of CLA may increase the cellular lipolysis,leading to decreased embryo developmental ability.In addition,diet supplemented with fish oil did not affect the developmental ability of oocytes where the Ca2+ homestasis was perturbed. |
| Key words: fish oil conjugated linoleic acid mice oocyte mitochondria blastocyst |
