| 摘要: |
| 为测定鼻气管炎鸟疫杆菌对不同药物的敏感程度,本试验从北京、吉林、河北、内蒙和山东地区患严重气囊炎病鸡的肺脏以及辽宁肉种鸡蛋黄中成功分离到了6株鼻气管炎鸟疫杆菌(Ornithobacterium rhinotracheale,ORT),并通过染色镜检、生化试验和PCR法对其进行了鉴定。随后选择山东分离株在培养基上繁殖,经腹腔注射接种21日龄SPF鸡,接种后出现典型的肺炎和气囊炎,并测定其半数致死量。在此基础上,采用微量测定法测定6个临床分离菌株和3个对照菌株对12种药物的最小抑菌浓度(MIC)。9株ORT对于莫西沙星、甲磺酸加替沙星和乳酸环丙沙星比较敏感,MIC值为0.49~31.25 μg/mL;临床广泛使用的盐酸强力霉素MIC测定值变化较大,分布在1.90~125 μg/mL;除北京株和吉林株外,其他7株ORT对利福平比较敏感,MIC值为3.91~15.6 μg/mL;分离株对于氟苯尼考、四环素、金霉素、磺胺间甲氧嘧啶、磷霉素钠和头孢噻肟钠不敏感,部分菌株的MIC值已经超过1 000 μg/mL。 |
| 关键词: 鸡 鼻气管炎鸟疫杆菌 分离 鉴定 最小抑菌浓度 |
| DOI：10.11841/j.issn.1007-4333.2012.02.021 |
| 投稿时间：2011-10-31 |
| 基金项目:中国农业大学动物医学院2010年URP计划资助 |
|
| Isolation and identification and minimum inhibiting concentration determination of Ornithobacterium rhinotracheale |
|
LIU Ai-jing, PAN Qing, TIAN De-yu, HOU Na, HE Cheng
|
| (College of Veterinary Medicine, China Agricultural University, Beijing 100193, China) |
| Abstract: |
| To study the sensentivity of Ornithobacterim thinotracheale (ORT) on different medicines,six isolates were used after confirmed by Gram-stain,biochemical test and PCR methods,where five samples were isolated from the lungs with the severe pneumonia in broilers in Beijing,Hebei,Inner Mongolia,Jilin and Shandong province,and one was obtained from the broiler breeder’s egg yolk in Liaoning.Subsequently,the typical pneumonia were replicated with the characterization of the airsaccitis in 21-day SPF chickens post infection intraperioneally with the Shandong isolate.Additionally,half lethal dose (LD50) was determined to be 1.43×108cfu/mL in SPF chickens.Furthermore,minimum inhibition concentrations (MIC) of nine isolates were determined post incubation with the different antibiotics,including 6 isolated strains and 3 reference strains.Nine ORT strains were more sensitive to moxifloxacin gatifloxacin,and ciproxacin and the average MIC arranged from 0.49 to 31.25 μg/mL.Although doxycycline was widely used against avian respiratory disease,the MIC was found to be diversity arranged from 1.90 to 125 μg/mL.As for the MIC of rifampcin,7 ORT strains was found to be sensitive and the MIC values arranged from 3.91 to 15.6 μg/mL except for the Beijing isolate and Jilin strain.Furthermore,9 isolates were not sensitive to the drugs like florfenicol,tetracycline,aureomycin,sulfamonomethoxine sodium,fosfomycin sodium and cefotaxime sodium,the MIC values was higher than 1 000.0 μg/mL. |
| Key words: chicken Ornithobacterium rhinotracheale isolation identification minimum inhibition concentration |
