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基于线粒体COⅠ基因的上海地区橘小实蝇种群遗传关系研究
吴广超1,2, 李云龙2,3, 李志红2, 柳丽君2
0
(1.上海市林业病虫防治检疫站, 上海 200072;2.中国农业大学 农学与生物技术学院, 北京 100193;3.北京市植物保护站, 北京 100029)
摘要:
利用线粒体DNA(mtDNA)序列分析技术,选用长为690 bp的COⅠ基因序列作为目的基因,通过对福建厦门、海南儋州、四川攀枝花、广东肇庆、云南思茅和上海宝山、嘉定、浦东、青浦、松江在内的共10个地理种群的122条橘小实蝇线粒体COⅠ基因序列和单倍型网络进化分析,揭示上海地区橘小实蝇的种群遗传结构和扩散规律。结果表明:橘小实蝇松江种群主要来自于云南思茅种群,另一部分来自于广东肇庆种群和上海嘉定种群,还有一部分来源于较云南思茅种群和海南儋州种群等更为原始的其他种群;青浦种群主要来源于云南思茅种群,另一部分来源于福建厦门种群和上海嘉定种群;宝山种群主要来源于云南思茅种群,另一部分可能来自于上海松江和广东肇庆;嘉定种群主要来源于云南思茅种群,另一部分可能来自于上海宝山;浦东种群主要来自于云南思茅种群和上海嘉定种群。
关键词:  橘小实蝇  线粒体DNA  COⅠ基因  种群遗传
DOI:10.11841/j.issn.1007-4333.2012.02.017
投稿时间:2011-07-20
基金项目:辰山植物园项目(11-11464)
The genetic relation of the population of Bactrocera dorsalis(Diptera:Tephritidae) in Shanghai District based on mtDNA COⅠ gene
WU Guang-chao1,2, LI Yun-long2,3, LI Zhi-hong2, LIU Li-jun2
(1.Forest Pest Management and Quarantine Station of Shanghai, Shanghai 200072, China;2.College of Agronomy and Biotechnology, China Agriculture University, Beijing 100193, China;3.Beijing Plant Protection Station, Beijing 100029, China)
Abstract:
Sequence analysis technology of mitochondrion DNA,a 690 bp COⅠ gene,was used in this study.By sequencing and the haplotype network evolution of the COⅠ gene from a total of 10 geographic populations with 122 COⅠ gene sequences from Xiamen,Danzhou,Panzhihua,Zhaoqing,Simao,and Shanghai Baoshan,Jiading,Pudong,Qingpu,Songjiang,the population genetic structure and expansion trend were analysed.The results showed that Bactrocera dorsalis(Hendel) populations of Songjiang mainly was spread from Simao Yunnan,the other some populations from Zhaoqing Guangdong and Jiading Shanghai populations.In addition, some Bactrocera dorsalis(Hendel) were from the population which were earlier than those of Simao,Yunnan and Danzhou Hainan.Qingpu population mainly was from Simao,Yunnan populations,some from Xiamen,Fujian,and Jiading,Shanghai populations.Baoshan population of Bactrocera dorsalis(Hendel) was also mainly from Simao,Yunnan populations, others from Songjiang,Shanghai and Zhaoqing Guangdong.Jiading populations of Bactrocera dorsalis (Hendel) were mainly from Simao,Yunnan populations,but another part from Baoshan Shanghai.Pudong population was mainly from Simao,Yunnan and Jiading Shanghai populations.
Key words:  Bactrocera dorsalis  mitochondrial DNA  COⅠ genes  population genetics