| 摘要: |
| 为加速木质纤维素类生物质资源的能源转化,本研究以能源作物柳枝稷为基质材料,筛选可以高效产酶的微生物复合系。通过长期的限制性筛选,获得一组有效降解柳枝稷的复合系,进而对复合系的酶学特性及微生物多样性进行了研究。在连续2周的液体培养过程中,柳枝稷的干物质损失主要发生在前4 d,且干物质损失率占总损失率的70%。在14 d培养过程中,柳枝稷的纤维素及半纤维素的降解率分别达到了50%和60%。整个培养过程中的羧甲基纤维素酶活及木聚糖酶活结果显示,2种酶活的最高值分别达0.21 和3.75 IU。2种酶的优势作用pH分别为5.0和6.0,优势作用温度为60 ℃。克隆文库结果显示复合系由真菌和细菌共同组成,86%的细菌近缘种为Achromobacter xylosoxidans,91%的真菌近缘种为Fusarium sporotrichioides。 |
| 关键词: 柳枝稷 复合系 纤维素酶 木聚糖酶 微生物多样性 |
| DOI：10.11841/j.issn.1007-4333.2012.02.012 |
| 投稿时间：2011-08-16 |
| 基金项目:中央高校基本科研业务费专项基金(DL11BA11); 大庆市科技计划项目(scyh-2011-95) |
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| Microbial diversity of a microbial community colonizing switchgrass and characteristics of producing enzymes |
|
YANG Hong-yan1,2, LI Yu-hua1, JIA Hong-bai1, WU Hao1, WANG Xiao-fen2, CUI Zong-jun2
|
| (1.College of Life Sciences, Northeast Forestry University, Harbin 150040, China;2.College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China) |
| Abstract: |
| To fasten energy conversion of lignocellulosic biomass,we used the switchgrass as the substrate to screen the microbial community which could produce hydrolysis enzymes.By the continuous enrichment,a community was obtained.The characteristics to produce enzymes and microbial diversity of the community were mainly investigated.During the fourteen-day liquid cultivation the dry weight loss drastically occurred at the first four days and amounted to 70% of the total loss.14 days after liquid cultivation,the degradation rate of the cellulose and hemicellulose amounted to 50% and 60%,respectively,and higher CMCase and xylanase activity were both detected in the liquid cultivation system.The highest values for the CMCase activity and xylanase activity during the cultivation reached 0.21 and 3.75 IU,respectively.The investigations on the enzyme characteristics indicated that the optimal pH for CMCase was 5.0 and that for xylanase was 6.0.The optimal reaction temperature of CMCase and xylanase was 60 ℃.The results from the cloning libraries showed that the relative species of most bacteria was Achromobacter xylosoxidans and the relative species of most fungi was Fusarium sporotrichioides. |
| Key words: switchgrass microbial community cellulase xylanase clone library |
