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| 小麦钙联蛋白基因TaCNX60.0的克隆与表达分析 |
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邢晶莉1,2, 王凤涛1, 安艳秋1, 关尔鑫1, 蔺瑞明1, 冯晶1, 郭玉华2, 徐世昌1
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| (1.中国农业科学院 植物保护研究所/植物病虫害生物学国家重点实验室,北京 100193;2.沈阳农业大学 农学院,沈阳 110866) |
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| 摘要: |
| 钙联蛋白(Calnexin)是一类结构和功能非常保守的分子伴侣,在调控生物代谢和Ca2+信号传导等方面发挥重要作用。本研究通过筛选Yr5近等基因系cDNA文库,分离获得1个钙联蛋白基因,将其命名为TaCNX60.0。序列分析发现,该cDNA片段包含1个1 921 bp的开放阅读框,推测其编码包含535个氨基酸残基的蛋白质。利用半定量RT-PCR分析该基因的表达谱,发现小麦受到植物激素ABA、低温和干旱胁迫处理后,TaCNX60.0的表达受到抑制,而条锈菌小种CYR32和白粉菌混合菌系侵染能诱导TaCNX60.0表达量增加,说明小麦钙联蛋白基因TaCNX60.0可能在植物防卫反应和抵抗逆境胁迫过程中发挥作用。 |
| 关键词: 小麦 钙联蛋白 分子伴侣 条锈菌 逆境胁迫 |
| DOI：10.11841/j.issn.1007-4333.2012.01.004 |
| 投稿时间：2011-04-12 |
| 基金项目:转基因生物新品种培育重大专项(2009ZX08009-053B); 国家自然基金国际合作项目(30821140351); 国家"973"计划项目(2011CB100403); 公益性行业专项(200903035) |
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| Molecular cloning of wheat calnexin gene TaCNX60.0and analyzing of its expression |
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XING Jing-li1,2, WANG Feng-tao1, AN Yan-qiu1, GUAN Er-xin1, LIN Rui-ming1, FENG Jing1, GUO Yu-hua2, XU Shi-chang1
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| (1.State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China;2.College of Agronomy,Shenyang Agricultural University,Shenyang 110866,China) |
| Abstract: |
| Calnexin is one of the molecular chaperone molecules with highly conserved structures and biological functions.It plays crucial roles in the regulation processes of biological metabolism and Ca2+ signal transduction.In this study,we screened a cDNA library of a near-isogenic line of wheat stripe rust resistance gene Yr5 and cloned a calnexin gene named TaCNX60.0.Sequence analysis showed that the cDNA fragment contained a 1 921-bp open reading frame,and was predicted to encode a protein with 535 amino acids.Semi-quantitative RT-PCR was performed to investigate the gene expression profiles when wheat plants were treated with different stress conditions and after inoculated with wheat pathogens.It was found that TaCNX60.0 expression was suppressed by exogenous phytohormone ABA,cold and drought treatments.However,it was induced after infection by a strain of Puccinian striiformis CYR32 and mixed races of Erysiphe graminis f. sp. triticis.These results indicated that TaCNX60.0 may involve in wheat defense and stress responses. |
| Key words: wheat calnexin molecular chaperone Puccinia striiformis abiotic stress |
