| 摘要: |
| 探讨不同pH模拟酸雨对葱兰主要生理特性的影响及Ca2+的调控作用。通过盆栽试验,研究10 mmol/L Ca(NO3)2处理后不同pH(2.0、2.5、3.0、4.0和5.6)模拟酸雨胁迫对葱兰的有机物代谢、膜系统稳定性、抗氧化酶活性、叶绿素(Chl)及气体交换参数的影响。结果表明:随着pH的降低,葱兰叶片相对电导率(γ)、丙二醛(MDA)和超氧化物歧化酶(SOD)逐渐升高,可溶性蛋白、可溶性糖含量和过氧化物酶(POD)逐渐下降;Chl和净光合速率(Pn)随pH的降低而降低,气孔导度(Gs)和胞间CO2浓度(ci)先随pH的降低而升高,当pH 2.0时显著降低;在pH≤4.0时,可溶性糖与pH呈显著正相关,SOD和Pn均与pH呈显著负相关。同一强度酸雨胁迫下,经10 mmol/L Ca(NO3)2处理后,葱兰叶片中可溶性蛋白、可溶性糖、POD、SOD、Chl和Pn均有不同程度的升高,γ和MDA显著降低,且各指标的变化随pH的降低变化不明显。模拟酸雨胁迫下,Ca2+能增强葱兰各项生理功能的稳定性,从而减轻酸雨对葱兰的伤害,试验还发现,Ca2+对葱兰的调控作用在强酸度(pH≤3.0)胁迫下,效果更明显。 |
| 关键词: 葱兰 模拟酸雨 钙调节 生理特性 |
| DOI：10.11841/j.issn.1007-4333.2011.06.017 |
| 投稿时间：2011-01-30 |
| 基金项目:四川农业大学"双支"计划 |
|
| Regulation functions of Ca2+ physiological of Zephyranthes candidaunder simulated acid rain stress |
|
YANG Hui, PAN Yuan-zhi
|
| (College of Landscape Architecture, Sichuan Agricultural University, Chengdu 611130, China) |
| Abstract: |
| The objective of this experiment is to study the effect of simulated acid rain at different pH levels on physiological of Zephyranthes candida Herb.and the regulation function of Ca2+.With pot experiments,this paper studies the effect of simulated acid rain that included 5 different pH levels (pH 2.0,2.5,3.0,4.0 and 5.6) and the regulation function of Ca2+ on the organic matter metabolism ,and membrane damage,and antioxidant enzyme activity,Chl content ,and gas exchange parameters of Zephyranthes candida leaves.With the decrease of the pH,value of REC,MDA and SOD,increased gradually while value of soluble protein,soluble sugar,POD,Chl and Pn decreased gradually,Gs and Ci were firstly increased and then significantly reduced in pH2.0;when pH≤4.0,there was a significant correlation between Soluble sugar-pH meanwhile there were significant correlations between SOD-pH and Pn-pH.However,under the same acid rain coercion in the leaves pretreated with 10 mmol/L Ca(NO3)2,soluble protein,soluble sugar,POD,SOD and Chl,Pn increased of different level,and REC,MDA reduced significantly;each index did not change significantly with the pH lowing.Under simulated acid rain stress,pretreated with Ca2+could strengthen various physiological function of Zephyranthes candida Herb.,thus reduced the damage by acid rain.Experiments have found that the regulation effects of Ca2+ was more apparent in pH≤3.0. |
| Key words: simulated acid rain Zephyranthes candida Herb. Calcium regulation physiological characteristics |
