| 摘要: |
| 通过microarray(微列阵芯片)分析,从珠眉海棠盐胁迫cDNA文库中分离得到盐诱导的MzDREBb基因全长cDNA序列,研究苹果属植物DREB转录因子在胁迫中的作用。结果表明:MzDREBb全长1 185 bp,开放阅读框共714 bp,5'-UTR和3'-UTR的长度分别是121和350 bp;MzDREBb编码237个氨基酸,有一个AP2/ERF结构域;系统分类将MzDREBb归入DREB家族的A-5亚组;MzDREBb定位在细胞核中,具有转录激活活性;半定量RT-PCR表明MzDREBb基因受到盐诱导。超表达MzDREBb基因的拟南芥耐盐能力增强。以上结果表明MzDREBb基因在盐胁迫应答中起重要作用。 |
| 关键词: 珠眉海棠 DREB转录因子 盐胁迫 功能研究 |
| DOI：10.11841/j.issn.1007-4333.2011.06.012 |
| 投稿时间：2011-03-09 |
| 基金项目:国家自然科学基金项目(30600416,31171940);北京市自然科学基金项目(5072027,6112012); 国家"973"计划项目(SQ2011CB016411) |
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| Cloning and characterization of salt-responding MzDREBb genefrom Malus zumi Mats |
|
HUANG Li-fang, LI Qing-tian, JIANG Yu-zhuang, CHEN Hao, SUN Yang-wu, KONG Jin
|
| (College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China) |
| Abstract: |
| The DREBs are transcription factors which play central roles in abiotic stress response.Malus zumi, an apple rootstock,can survive 0.6% NaCl.The full-length cDNA sequence of MzDREBb gene was isolated from a cDNA library of Malus zumi by microarray analysis.The full-length cDNA of MzDREBb was 1 190 bp.Its open reading frame of 714 bp encoded a protein of 237 amino acids.The 5'-UTR and the 3'-UTR were 121 and 355 bp respectively.Base on multiple sequence alignment and phylogenetic characterization,MzDREBb was classified into A-5 subgroup of the DREB subfamily.The MzDREBb was localized in the nucleus,which exhibited transactivation activity in yeast two-hybrid system.MzDREBb was induced by either stress of high salinity or low temperature,but not by abscisic acid (ABA).Transgenic Arabidopsis over-expressing MzDREBb could enhance salt tolerance.In conclusion,MzDREBb is involved in salt response through an ABA-independent pathway in Malus zumi. |
| Key words: Malus zumi Mats MzDREBb salt stress functional research |
