| 摘要: |
| 通过研究3种水稻种子寄藏真菌检测方法的异同和适应性,为提高水稻种子健康检测的针对性提供科学依据。以云南省具有代表性的3个主栽水稻品种楚粳26、滇优35和宜香9的种子为材料,采用洗涤法、PDA平板法进行种子寄藏真菌检测;同时采用滤纸保湿法对此3个品种以及富优80等产地海拔高度不同的共计20个水稻品种的种子进行检测。结果显示,洗涤法适用于种子外部带菌检测,洗涤时间为20 min,洗涤液稀释10倍涂平板可以观测记录带菌的数量,供试水稻种子外部主要携带Aspergillus、Fusarium、Penicillium、Alternaria,它们出现的比例分别为21.4%、17.9%、16.7%和8.3%;PDA平板法可以满足整粒种子以及解剖后种子不同部位的寄藏真菌检测,结果与洗涤法基本一致,种子携带4个属真菌的分离比率分别为42.7%、25.6%、1.2%和4.9%;滤纸保湿法可较好地检测20个水稻品种整粒种子的带菌情况,共检测到24个属的真菌,主要优势菌为Aspergillus、Fusarium和Penicillium,它们分别在14、19和18个供试品种中广泛分布,出现频率最大,而在版纳10号等少数地方种水稻种子中,上述3个属的真菌未检出或者检出频率明显降低。研究分析了种子类型、来源、产地海拔高度与种子寄藏真菌种类的关系,提出了对3种检测方法中获得的未知真菌分类地位进行进一步鉴定的建议。 |
| 关键词: 水稻 主栽品种 种子健康 寄藏真菌 检测方法 对比分析 |
| DOI：10.11841/j.issn.1007-4333.2011.06.007 |
| 投稿时间：2011-04-11 |
| 基金项目:云南省科技创新强省计划项目省院省校科技合作专项(2008AD010) |
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| Comparative analysis of three kinds of testing methodsfor rice seed borne fungi |
|
LI Xiao-lin1,2, GU An-yu1,2, ZHANG Xin-yu1, XU Yu-ran2, LUO Lai-xin1, LI Jian-qiang1
|
| (1.College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China;2.Institute of Food Crops, Yunnan Academy of Agricultural Sciences, Kunming 650205, China) |
| Abstract: |
| The differences and applicability of three methods for rice seed borne fungi testing were studied to provide scientific references to improve the accuracy of rice seed health testing.The seed borne fungi of japonica rice variety Chujing26,Dianyou35 and Yixiang9 which are mainly cultivated in Yunnan province were studied by using both washing test and PDA culture plate methods,and at the same time,the blotter moisture-retaining culture method was used to detect the seed born fungi of 20 rice hybrids,including Chujing26,Dianyou35,and Yixiang9 which were from production areas with different altitude.The results showed that washing test was applicable to detect the fungi on seed surface,the washing time was 20mins,and the washing solution with dilution ten times could be directly applied to PDA culture plate for fungi observation and counting.The Aspergillus,Fusarium,Penicillium,and Alternaria were mainly isolated from the external parts of rice seeds tested and their isolation frequency respectively were 21.4%,17.9%,16.7%,and 8.3%.PDA culture plate method was applicable to detect seed born fungi from one whole kernel or different parts of rice seed,and the test results were basically same with the results from wash test,the four major genera of fungi isolation frequency respectively were 42.7%,25.6%,1.2%,and 4.9%.There were totally 24 genera of fungi were detected from 20 rice hybrid seeds by the blotter moisture-retaining culture method,the dominant fungi were Aspergillus,Fusarium,and Penicillium which were respectively detected from 14,19 and 18 hybrids of rice seed and with high isolation frequency and widely distribution,but those three kinds of genera were failed to be detected or detected with very low isolation frequency from the seed of Banna10 and a few local rice varieties.The correlation between seed types,sources,altitude of production area,and the kinds of seed born fungi were analyzed to provide further references to identify and classify the unknown fungi isolated by above washing test,PDA culture plate and blotter moisture-retaining culture methods. |
| Key words: rice mainly cultivated varieties seed health seed borne fungi testing method comparative analysis |
