| 摘要: |
| 研究芦丁对哺乳母鼠乳腺发育、泌乳相关激素水平及其受体表达量的影响。试验选择18只Wistar哺乳母鼠,随机分为3组,分别为对照组(Lac-Con组)每日每只灌喂生理盐水(2 mL)1次;芦丁组(Lac-Rut组)每日每只灌服芦丁(60 mg/kg,BW)1次;雌二醇组(Lac-Est组)每周每只肌注(60 μg/kg,BW)1次;从哺乳第4天开始连续给药2周,基础日粮相同。检测哺乳母鼠血浆与乳腺组织中雌激素(雌二醇,E2)、孕激素(P)、催乳素(PRL)和生长激素(GH)的含量,乳腺组织中雌激素受体(雌激素α受体,ERα)、孕激素受体(PR)、催乳素受体(PRLR)和生长激素受体(GHR)的表达量,并进行乳腺组织切片组织形态学观察。试验显示:E2、PRL和GH在血浆与乳腺组织里的含量都表现为Lac-Est组>Lac-Rut组>Lac-Con组(P<0.05);P含量组间差异不显著(P>0.05);ER、PR、PRLR和GHR在乳腺组织中的表达量均为Lac-Est组>Lac-Rut组>Lac-Con组(P<0.05);乳腺腺泡/管腔直径统计显示Lac-Est组和Lac-Rut组高于Lac-Con组(P<0.05),Lac-Est组与Lac-Rut组差异不显著(P>0.05)。试验结果表明,芦丁能够提高哺乳母鼠体内E2水平,促进PRL和GH分泌,诱导ER、PR、PRLR和GHR的表达,具有弱雌激素样作用功效。 |
| 关键词: 芦丁 大鼠 激素 受体 |
| DOI：10.11841/j.issn.1007-4333.2011.05.016 |
| 投稿时间：2011-03-15 |
| 基金项目:国家"十一五"科技支撑计划项目(2006BAD12B04-04) |
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| Effect of dietary rutin on mammary gland development and the leveland receptors of estrogen in lactation rats |
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GUO Xu-dong1, DIAO Qi-yu1, WANG Yue-ying2, TU Yan1, YAN Gui-long3, WANG Xin-jian2, LI Jun-tao2, DENG Dong-xiao2
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| (1.Feed Research Institute/Key Laboratory of Feed Biotechnology of Ministry of Agriculture,Chinese Academy of Agricultural Sciences,Beijing 100081,China;2.Key Laboratory of Animal Growth and Development,Ministry of Agriculture,Henan Agricultural University,Zhengzhou 450002,China;3.College of Animal Science and Technology,Hebei North University,Zhangjiakou 075000,China) |
| Abstract: |
| The objective of this study was to observe effects of a natural plant extract rutin in mammary gland development,endocrine hormone levels and receptor expression were measured for lactation rats.Eighteen Wistar rats were randomly assigned to three treatments:control (Lac-Con) group was treated with gastric infusion of 2 mL normal saline per mouse per day;Rutin (Lac-Rut) treatment group was treated with gastric infusion of rutin 60 mg/kg BW per day;and Estradiol (Lac-Est) group was treated with intramuscular injection of estradiol 60 μg/kg BW weekly started from the fourth lactation day and continue for two weeks with the same basal diet as control group.The level of estrogen (E2),progesterone (P),prolactin (PRL) and growth hormone (GH) in plasma and gland tissues were detected;and the expression of estrogen receptor (ER),progesterone receptor (PR),prolactin receptor (PRLR) and growth hormone receptor (GHR) in gland tissues were measured.Histomorphology of development about mammary gland were observed.(Results)The results showed that,the relationship among the level of E2,PRL and GH in plasma and gland tissues was Lac-Est>Lac-Rut>Lac-Con (P<0.05),the level of P in plasma and gland tissues was not significant among groups (P>0.05).The expression of ER,PR,PRLR and GHR in gland tissues was Lac-Est>Lac-Rut>Lac-Con (P<0.05).The diameter of mammary gland alveolus showed that Lac-Est and Lac-Rut were higher than Lac-Con (P<0.05),there was not significant between Lac-Est and Lac-Rut (P>0.05).The test indicated that rutin could improve the level of E2 in lactation rats,increase PRL and GH release,up-regulated the expression level of ER,PR,PRLE and GHR.And finally rutin had weakly estrogen-like effect. |
| Key words: rutin rat hormone receptor |
