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| 一组秸秆分解菌群的稳定性及对还田秸秆的促腐效果 |
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李培培1,2, 韩宝文3, 曹燕篆1, 李佳佳1, 王小芬1, 崔宗均1
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| (1.中国农业大学 农学与生物技术学院/生物质工程中心,北京 100193;2.河南农业大学 资源与环境学院,郑州 450002;3.河北省农林科学院 农业资源环境研究所,石家庄 050051) |
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| 摘要: |
| 探讨一组秸秆还田菌群ADS-3的功能稳定性及初步观察其接种于土壤后对还田秸秆的促腐效果。结果表明:在培养瓶中菌种ADS-3对小麦、水稻和玉米3大农作物秸秆都有稳定高效的分解能力,35 ℃条件下培养11 d,能分解稻秆51.6%、小麦秸秆44.7%、玉米秸秆40.8%;在4、15和35 ℃条件下,尽管ADS-3对小麦秸秆的分解率差异很大,依次为6.8%、21.6%和44.7%;但是用变性梯度凝胶电泳(DGGE)检测其培养液,发现不同温度条件下ADS-3的微生物组成稳定。将菌种接种到模拟秸秆还田土壤中,初步试验结果表明ADS-3能显著提高秸秆的腐解速度(P<0.05)。试验结果为该菌群进一步开发秸秆还田促腐菌剂提供试验参数和依据。 |
| 关键词: 秸秆 菌群 接种 |
| DOI：10.11841/j.issn.1007-4333.2011.05.008 |
| 投稿时间：2010-03-10 |
| 基金项目:国家"十一五"支撑计划"沃土工程"项目(2006BAD25B04); 公益性行业科研专项(200803033) |
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| Functional stability and straw-degrading enhancementof a microbial community |
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LI Pei-pei1,2, HAN Bao-wen3, CAO Yan-zuan1, LI Jia-jia1, WANG Xiao-fen1, CUI Zong-jun1
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| (1.Center of Biomass Engineering/College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,China;2.College of Resources and Environment,Henan Agricultural University,Zhengzhou 450002,China;3.Institute of Resource and Environment,Hebei Academy of Agriculture and forestry Sciences,Shijiazhuang 050051,China) |
| Abstract: |
| The functional stability of a microbial composite community ADS-3 and its effect on straw degrading enhancement after straw returning to field were investigated.The results indicated that ADS-3 had high degrading capacity for three privileged crop straws in flask,with 51.6% for rice straw,44.7% for wheat straw and 40.8% for corn straw 11 days after incubation.Although ADS-3 could degrade 6.8%,21.6% and 44.7% of wheat straw under temperatures of 4,15 and 35 ℃ respectively,polymerase chain reaction (PCR) denaturing gradient gel electrophoresis (PCR-DGGE) revealed its microbial component was rather stable.Wheat straw degrading enhancement was significantly improved by inoculating straw-amended soil with ADS-3 (P<0.05).This study provided test parameters for further field experiment and agent preparation. |
| Key words: straw microbial community inoculated |
