| 摘要: |
| 为探讨不同生理状态热应激奶牛嗜淀粉瘤胃杆菌(Ruminobacter amylophilus)的含量差异,选择48头产奶母牛,按照因子试验设计分为泌乳中期和后期牛、高产和中产牛、初产和经产牛。奶牛经历1周热应激后,采集瘤胃液,利用RT-PCR定量分析方法,测定瘤胃液中嗜淀粉杆菌的含量。结果显示,微生物的数量经lg转化后,泌乳中期奶牛嗜淀粉瘤胃杆菌为7.70,泌乳后期为7.95,差异不显著(P＞0.05);高产牛嗜淀粉瘤胃杆菌为7.46,中产牛为8.20,差异不显著(P＞0.05);初产牛嗜淀粉瘤胃杆菌为7.97,经产牛为7.68,差异不显著(P＞0.05)。综上所述,在奶牛遭受热应激时,嗜淀粉瘤胃杆菌含量与奶牛的耐受热应激能力无明显关系。 |
| 关键词: 奶牛 瘤胃 热应激 嗜淀粉杆菌 实时定量PCR |
| DOI：10.11841/j.issn.1007-4333.2011.04.016 |
| 投稿时间：2011-01-10 |
| 基金项目:"十一五"国家奶业科技支撑计划(2006BAD04A14); 河南科技大学博士基金资助 |
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| Quantities of ruminal cellulytic bacteria in heat stress dairy cattle |
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WANG Jian-ping1, WANG Jia-qi2, BU Deng-pan2
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| (1.School of Livestock Science & Technology,Henan University of Science & Technology,Luoyang 471003,China;2.Institute of Animal Science Chinese Academy Agricultural Sciences,Beijing 100193,China) |
| Abstract: |
| The objective of this study was to investigate the quantities of Ruminobacter amylophilus in lactating dairy cows under heat stress.Forty eight cows were assigned to mid-stage or late-stage,high producing or medium producing,primiparous or multiparous groups with a factorial design.Rumen fluid was sampled 5 h in the morning post-feeding via oral cavity with stomach-tube when the animals had suffered heat stress for one week.The real-time PCR was employed with the specific primers targeting 16s rDNA genes to test the abundance of Ruminobacter amylophilus.The quantities expressed as a log-transformed of Ruminobacter amylophilus was 7.70 and 7.95 in mid-stage and late-stage dairy cows (P＞0.05),7.46 and 8.20 in high and medium producing cows (P＞0.05),7.97 and 7.68 in primiparous and multiparous,respectively.In summary,the quantities of Ruminobacter amylophilus has no relationship with heat resistence of cow. |
| Key words: dairy cows heat stress cellulytic bacteria RT-PCR |
