| 摘要: |
| 为深入了解黑腹果蝇CG42741基因的表达及其编码蛋白质的结构,利用半定量和生物信息学的方法对其进行了详细的研究。RT-PCR结果显示黒腹果蝇CG42741基因在胚胎期、幼虫期和蛹期均有表达,但表达量比较低,只是在蛹期表达量相对升高。此外,通过生物信息学的方法对果蝇中CG42741基因所编码的蛋白质进行了结构与功能的预测。结果显示,CG42741蛋白定位于细胞核内,其羧基末端含有3个串联的C2H2类型的锌指结构;它属于KLF家族蛋白,是一个转录调控因子,能够与富含GC的基因组序列特异结合,其功能可能与细胞的生长、增殖有关。 |
| 关键词: 黒腹果蝇 蛋白质结构预测 CG42741基因 RT-PCR KLF家族 |
| DOI：10.11841/j.issn.1007-4333.2011.04.014 |
| 投稿时间：2010-12-03 |
| 基金项目:国家自然科学基金资助项目(30771535) |
|
| Structural analysis and functional prediction of CG42741protein in Drosophila melanogaster |
|
YU Bo, XIN Yi, ZHAO Chun-jiang, WU Chang-xin, DENG Xue-mei
|
| (College of Animal Science and Technology,China Agricultural University,Beijing 100193,China) |
| Abstract: |
| In this study,the CG42741 gene expression were found at embryonic,larval and pupal stage by RT-PCR,but such expression was very low at all of the three stages.Comparative to embryonic and larval stages,the expression of the gene was higher at pupal stage.The structural analysis and functional prediction of CG42741 protein also conducted in Drosophila melanogaster.Our data showed that this protein was characterized by three Cys2His2 zinc fingers located at the C-terminus and executed its function in nucleus.The protein belonged to the Kruppel-like family of transcription factors and could bind with GC-rich sites in genomic DNA.And we inferred the CG42741 protein might play an important role in cell proliferation. |
| Key words: Drosophila melanogaster protein structure prediction CG42741 gene RT-PCR Kruppel-like factors |
