| 摘要: |
| 为人工调控花色苷合成,以‘京优’葡萄为实验材料,应用液相色谱-质谱(HPLC-MS)技术和荧光定量PCR,研究了萘乙酸(NAA)和脱落酸(ABA)处理对葡萄果皮花色苷积累及其生物合成相关基因表达的影响。结果表明:在‘京优’葡萄果皮中,可检测到16种花色苷;ABA处理的花色苷含量高于对照,NAA处理低于对照,并且ABA处理的果皮比对照和NAA多出3种花色苷;葡萄果实发育进入转色期,花色苷合成过程中类黄酮途径的结构基因上调表达,完熟后下调表达;ABA处理的果皮花色苷结构基因相对表达量高于对照,而NAA则低于对照。外源ABA促进花色苷积累及其合成相关基因的表达,而NAA则抑制其表达。 |
| 关键词: 葡萄 花色苷 脱落酸 萘乙酸 定量PCR |
| DOI：10.11841/j.issn.1007-4333.2011.04.005 |
| 投稿时间：2010-11-30 |
| 基金项目:现代农业产业技术体系建设专项资金(CARS-30) |
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| Effects of NAA and ABA treatments on the expression of anthocyaninbiosynthetic genes in 'Jing You' grape berry skin |
|
ZHOU Li1,2, WANG Jun1,3
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| (1.Key Laboratory of Forest Tree Genetic Improvement and Biotechnology of Ministry ofEducation/College of Forestry,Northeast Forestry University,Harbin 150040,China;2.Qiqihaer Forestry School,Qiqihaer 161006,China;3.Center for Viticulture and Enology,College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China) |
| Abstract: |
| In order to control anthocyanin synthesis of 'Jingyou' grape,we applied high performance liquid chromatography-mass spectrometry (HPLC-MS) and real time reverse transcription-polymerase chain reaction (RT-PCR) for studying the effects of naphthaleneacetic aicd (NAA) and abscisic acid (ABA) treatments on the accumulation of anthocyanins,as well as the expression of anthocyanin biosynthetic correlative genes.The results showed that there were 16 sorts of anthocyanins in 'Jing You' berry skin.The anthocyanin contents of ABA treatment were higher but NAA treatment lower than that of control.And there were three extra anthocyanins in ABA treatment of berry skin.The structural genes in flavonoid pathway of anthocyanin synthesis were up-regulated at veraison,but down-regulated after complete maturity.The expression of anthocyanin structural genes was higher in ABA treatment than control,but lower for NAA treatment than control.The accumulation of anthocyanin and the correlative genes of anthocyanin synthesis were promoted by exogenous ABA.And NAA treatment had the opposite impacts. |
| Key words: grape anthocyanin ABA NAA RT-PCR |
