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玉米穗高系数的遗传分析与QTL定位研究
蒋锋1, 刘鹏飞1, 王汉宁2, 张金凤1, 王晓明1
0
(1.仲恺农业工程学院 农学院,广州 510225;2.甘肃农业大学 农学院,兰州 730070)
摘要:
选用穗高系数有显著差异的甜玉米自交系T14和T4为亲本配制杂交组合,用主基因+多基因混合遗传模型和P1、P2、F1、B1、B2和F2共6个世代联合分析的方法对穗高系数性状进行分析;以330个F2单株为分离作图群体,用复合区间作图法在F2和F2:3家系中检测穗高系数QTL。通过研究玉米穗高系数的遗传模式和QTL定位,为玉米高产、耐密和抗倒伏育种提供有价值的理论依据。结果表明:玉米穗高系数受1对加性主基因+加性-显性多基因控制,在各个分离世代都以主基因遗传为主。在F2群体和F2:3家系中分别定位到4个和6个穗高系数QTL;其中有3个QTL在F2群体和F2:3家系中均可被检测到。检测到的高贡献率的主效QTL都集中在第1染色体上,形成1个明显的穗高系数QTL集中区,这一结果与1对主基因的遗传模型相吻合。试验得出的玉米穗高系数的遗传模式和QTL,可为加快高产、耐密和抗倒伏育种进程及实现分子标记辅助选择提供有力的研究基础。
关键词:  玉米  穗高系数  遗传模型  QTL
DOI:10.11841/j.issn.1007-4333.2011.04.002
投稿时间:2010-12-07
基金项目:国家科技支撑计划项目(2007BAD52B08); 广州市科技支撑计划项目(2008Z1-E541)
Genetic analysis and QTL mapping for ear height coefficient in corn
JIANG Feng1, LIU Peng-fei1, WANG Han-ning2, ZHANG Jin-feng1, WANG Xiao-ming1
(1.College of Agronomy,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;2.College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China)
Abstract:
To obtain the theoretical basis for breeding high yield and lodging-resistant corn under high density cultivation condition,genetic model and mapping for corn ear-hight-coefficiency (EHC) was investigated.Cross combination was designed by selecting two sweet corn inbred (T14 and T4) with significantly different EHC.Genetic segregation analysis for EHC was evaluated using major gene-polygene mixed inheritance models and joint analysis of P1,P2,F1,B1,B2 and F2 populations.A genetic mapping population of 330 F2 individuals was used to establish QTL for EHC with the screening method of composite interval mapping (CIM).EHC of T14 was controlled by a major additive gene and some additive-dominant polygenes,and inheritance of the major gene was dominant.Four QTLs were identified in F2 population and six in F2:3.Moreover,three QTLs were detected in both F2 and F2:3.The genetic model of one major gene is consistent with QTL mapping result that QTL with high contribution rate for EHC was clustered simultaneously on chromosome 1.The genetic mode and major QTL for EHC from this study may improve the efficiency of high yield and lodging resistance breeding and molecular marker-assisted selection (MAS) under high density cultivation condition.
Key words:  corn  ear height coefficient  genetic model  QTL