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| 微生物检测中糖量子点的制备及其与凝集素的相互作用 |
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孔潇艺1,2, 程水红2, 刘雪峰3, 李学兵2, 吴培星4
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| (1.天津科技大学 食品工程与生物技术学院,天津 300457;2.中国科学院 微生物研究所/中科院病原微生物与免疫学重点实验室,北京 100101;3.国家纳米科学中心,北京 100190;4.中国农业科学院 兰州畜牧与兽药研究所,兰州 730050) |
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| 摘要: |
| 为了以量子点为探针建立简便快速的病原微生物检测方法,采用配体交换法将具有微生物特异性的糖链偶联到CdSeS/ZnS量子点表面,制备2种糖量子点复合材料(甘露糖-量子点和半乳糖-量子点)。通过对它们的结构、物性和光谱学特性进行表征,结果显示:2种糖量子点均具有良好的水溶性、荧光发射和紫外-可见光吸收性能。进一步以这2种糖量子点为探针与凝集素共孵育后,溶液颜色和荧光发射光谱的变化表明:甘露糖-量子点对刀豆蛋白具有特异性,最低可检测浓度为95 nmol/L;而半乳糖-量子点对蓖麻毒素具有特异性,最低可检测浓度为3 nmol/L。因为细菌和病毒表面的粘附素多为凝集素类蛋白,本研究为进一步建立病原微生物的检测方法奠定了基础。 |
| 关键词: 微生物检测 量子点 糖 复合材料 凝集素 |
| DOI：10.11841/j.issn.1007-4333.2011.04.001 |
| 投稿时间：2011-02-14 |
| 基金项目:国家自然科学基金资助项目(30770486); 中国科学院知识创新工程重要方向性项目(KSCX2-YW-G-032, KSCX2-YW-R-178) |
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| Preparation of glyco-quantum dots for microbial detection |
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KONG Xiao-yi1,2, CHENG Shui-hong2, LIU Xue-feng3, LI Xue-bing2, WU Pei-xing4
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| (1.College of Food Engineering and Biotechnology,Tianjin University of Science & Technology,Tianjin 300457,China;2.Key Laboratory of Pathogenic Microbiology and Immunology/Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China;3.National Center of Nanoscience and Technology,Beijing 100190,China;4.Lanzhou Institute of Animal Husbandry and Veterinary Drug,Chinese Academy of Agricultural Sciences,Lanzhou 730050,China) |
| Abstract: |
| Carbohydrates with the binding capability to microbes were coupled with luminescent quantum dots(QDs) for microbial detections.Mannose and galactose-coated QDs(Man-QDs and Gal-QDs) were prepared using the thiolated sugars to replace the original ligands(mercaptoacetic acid) on the surface of CdSeS/ZnS QDs,according to a ligand exchange method.Structural and spectral characterizations of the resulting glyco-QDs revealed intense luminescent emission and UV-visible absorption,as well as high solubility and stability in water.Further studies on interactions between the glyco-QDs and corresponding lectins(ConA and RCA120) demonstrated that luminescent aggregations of the lectins caused by the multiple and specific bindings with the glyco-QDs could occur at nanomolar concentrations in a very short time(90 min),This indicated the potentials of the glyco-QDs for rapid and sensitive biosensings.As the lectin-like adhesins are commonly found on the surfaces of many kinds of microbes,the present work provides a baseline for the future studies on microbial detections with the glyco-QDs probes. |
| Key words: microbial detection quantum dot carbohydrate conjugate lectin |
