| 摘要: |
| 采用常规压片法,对百合21个栽培品种的染色体进行核型分析,结果表明:所试验品种染色体数均为2n=24。染色体呈现多态性,大部分品种都是由近中部(sm)、近端部(st)和端部(t)着丝粒染色体组成。亚洲百合、东方百合的染色体相对长度变动范围分别为5.1%~14.39%和5.11%~13.66%;最长与最短染色体的比值分别为2.09~2.61和2.01~2.64;核型不对称系数分别在78.54%~84.05%和77.04%~86.08%。21个品种核型类型有13个为"3B"型,8个为"4B"型;17个有随体的品种中,仅6个具有中间随体,其余均为端部随体;可以通过不同类型染色体数量以及它们在核型中排序位置的差异、随体的位置与数量差异对21个百合品种做早期鉴定。 |
| 关键词: 百合 栽培品种 染色体 核型分析 |
| DOI：10.11841/j.issn.1007-4333.2011.03.017 |
| 投稿时间：2010-09-03 |
| 基金项目:北京市科技新星项目资助(2003B013); 北京市教委科技创新平台项目资助(PXM2009-014207-078529) |
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| Karyotype analysis of 21 cultivars in Lilium |
|
YANG Xue-zhen1, ZHANG Ke-zhong1, JIA Yue-hui1, FAN Jia-lin1, LIU Shen2
|
| (1.College of Landscape,Beijing Agricultural College,Beijing 102206,China;2.State Key Laboratories for Agrobiotechnology,China Agricultural University,Beijing 100193,China) |
| Abstract: |
| The karyotypes of 21 lily cultivars were studied using traditional squash method.The results showed that the chromosome number of all tested cultivars was 24.Diversity of chromosome morphology was observed.Submetacentric acrocentric and telocentric chromosomes coexisted in most cultivars.The chromosome relative length varied from 5.1% to 14.39% and 5.11% to 13.66% in Asiatic Hybrids and Oriental Hybrids respectively.The ratio of the length of the longest chromosome to that of the shortest one varied from 2.09 to 2.61 and 2.01 to 2.64 respectively.The AS.K of the tested Asiatic cultivars and Oriental cultivars were 78.54%~84.05% and 77.04%~86.08% respectively.Of the 21 tested cultivars, 13 cultivars belonged to the stebbins ‘3B’ karyotype,the rest 8 cultivars belonged to the stebbins ‘4B’ karyotype. 6 of 17 cultivars with satellites were intermediate satellites,the other 11 were telocentric satellites.21 cultivars could be discriminated by means of the number and position of different kinds of chromosomes as well as the number and position difference of satellite in different cultivars. |
| Key words: Lilium cultivars chromosome karyotype analysis |
