| 摘要: |
| 本研究克隆了1个陆地棉新的钙依赖蛋白激酶基因GhCPK5(Genbank登录号为:HM002634)。GhCPK5基因组DNA序列全长3 031 bp,包含一个1 707 bp的开放阅读框,具有7个外显子和6个内含子。GhCPK5可能定位于细胞核内,有568个氨基酸残基,分子量为63 ku。氨基酸序列分析表明,陆地棉GhCPK5与杨树PtCPK5同源性达到89%,同样包含完整的激酶结构域和4个EF-hand结构域。RT-PCR分析表明,该基因在根、茎、叶等组织中表达;在盐胁迫处理下,GhCPK5在各组织的表达量明显上升。 |
| 关键词: 棉花 GhCPK5 盐胁迫应答 RACE 序列分析 |
| DOI：10.11841/j.issn.1007-4333.2011.03.002 |
| 投稿时间：2011-01-25 |
| 基金项目:国家"863"计划项目(2006AA10A108); 教育部新世纪优秀人才支持计划项目(NCET-06-0106) |
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| Isolation and sequence analysis of salt responsive geneGhCPK5 from Gossypium hirsutum L. |
|
ZHEN Jun-bo1, ZHANG Xi1, WANG Yu-mei2, ZHAI Zhi-xi1, LI Zhao-hu1, HUA Jin-ping1
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| (1.College of Agronomy and Biotechnology/Key Laboratory of Crop Genomics and Genetic Improvement of Ministry ofAgriculture/Beijing Key Laboratory of Crop Genetic Improvement,China Agricultural University,Beijing 100193,China;2.Institute of Cash Crops,Hubei Academy of Agricultural Sciences,Wuhan 430064,China) |
| Abstract: |
| In this study,a salt responsive gene GhCPK5 was firstly obtained from upland cotton using RT-PCR and RACE.GhCPK5 located probably in cell nucleus and the genomic sequence indicated that GhCPK5 had 7 exons and 6 introns.GhCPK5 contained an open reading frame (ORF) of 1 707 bp,which encoded a protein with 568 amino acids,with a molecular weight of 63 ku.GhCPK5 contained one protein kinase domain and four conserved EF-hand domains.The amino acid sequence of GhCPK5 shared 89% identify with Populus trichocarpa homolog PtCPK5.RT-PCR analysis revealed that GhCPK5 expressed in root, stem and leaf, and its expression level was significantly up-regulated by salt stress. |
| Key words: cotton GhCPK5 salt stress response RACE sequence analysis |
