| 摘要: |
| 试验应用气相色谱技术,以抗寒能力不同的园林树木华北和金山绣线菊为材料,通过低温与脱锻炼处理,研究低温诱导时其膜脂肪酸种类与含量的变化,探讨绣线菊细胞膜膜脂肪酸变化与抗寒能力的关系。结果表明:华北和金山绣线菊膜脂肪酸主要由豆蔻酸(14∶0)、棕榈酸(16∶0)、油酸(18∶1)、亚油酸(18∶2)、亚麻酸(18∶3)等组成,约占膜脂总脂肪酸的90%以上;饱和膜脂肪酸以棕榈酸(16∶0)为主,不饱和膜脂肪酸以亚油酸(18∶2)和亚麻酸(18∶3)为主;低温处理下金山绣线菊不饱和膜脂肪酸含量变化显著,华北绣线菊不饱和膜脂肪酸含量变化不显著,其较高的抗寒能力可能与低温下较稳定的膜脂不饱和度(IUFA)水平有关,维持华北绣线菊较稳定的膜脂不饱和度水平,需要一定的低温水平;低温处理对华北和金山绣线菊饱和膜脂肪酸含量也有一定影响。 |
| 关键词: 绣线菊 低温锻炼 脱锻炼 膜脂肪酸 抗寒能力 |
| DOI：10.11841/j.issn.1007-4333.2011.02.008 |
| 投稿时间：2010-06-02 |
| 基金项目:黑龙江省自然科学基金项目(C2007-11); 黑龙江省博士后基金项目(LBH-Z09288); 黑龙江省教育厅自然科学项目(11551040); 东北农业大学博士启动基金项目; 第48批中国博士后面上项目(415152) |
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| Changes of membrane fatty acid in 2 species Spiraea L.underlow-temperature and de-acclimation conditions |
|
LIU Hui-min, XING Yan-qiu, LV Gui-e, ZHU Yu-tao, YAN Yong-qing
|
| (College of Horticulture,North-east Agricultural University,Harbin 150030,China) |
| Abstract: |
| In order to understand the relationship between the changes of membrane fatty acid of Spiraea and its cold resistance,the garden trees Spiraea fritschiana Schneid.and Spiraea bumalda 'Goldmound’ with different cold resistance were used as materials,applying gas chromatography technology,designing low-temperature and deacclimation treatment,studying the species and the contents changes of their membrane fatty acid under low-temperature treatment.The results showed that the membrane fatty acid in S.fritschiana Schneid.and S.bumalda'Goldmound’ were mainly composed of myristic acid (14∶0),palmitic acid (16∶0),oleic acid (18∶1),linoleic acid (18∶2) and linolenic acid (18∶3).They composed more than 90% of total membrane fatty acid,saturated fatty acid was mainly composed by palmitic acid (16∶0),unsaturated fatty acid was mainly composed by linoleic acid (18∶2) and linolenic acid (18∶3).The unsaturated membranes fatty acid contents in S.bumalda 'Goldmound’ changed significantly under low temperature treatment,the unsaturated membrane fatty acid contents in S.fritschiana Schneid.changed non-significantly while the same treatment,its stronger cold-resistance might be related to the IUFA level that was more stable under low temperatures.A certain low-temperature level was needed in order to maintain a more stable unsaturation membrane fatty acid level to S.fritschiana Schneid.There was a certain influence to the saturated membrane fatty acid contents in S.fritschiana Schneid.and S.bumalda 'Goldmound’ under low temperature treatment. |
| Key words: Spiraea L. cold acclimation de-acclimation membrane fatty acid cold-resistance |
