| 摘要: |
| 番茄(Solanum lycopersicum L.)基因组序列的出现为开发InDel标记提供了基础。本研究选用18个InDel和22个SSR标记对我国的59个和美国的23个鲜食番茄品种的基因组DNA多态性进行比较分析,以探讨InDel标记在分子育种中应用的可行性。结果表明:在82个品种中InDel标记比SSR标记的多态性低;InDel与SSR标记扩增到的条带数在我国的品种中差异显著,但在美国的品种中差异不显著;InDel标记扩增到的条带数在我国的品种和美国的品种间差异极显著。聚类分析发现我国育成的番茄品种间遗传差异非常小,而我国品种与国外品种间遗传差异较大。 |
| 关键词: 番茄 InDel SSR 标记多态性 基因组DNA多样性 |
| DOI：10.11841/j.issn.1007-4333.2011.02.006 |
| 投稿时间：2010-06-11 |
| 基金项目:北京市果类蔬菜产业创新团队资助; 新世纪优秀人才支持计划(NCET-08-0531) |
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| Genomic DNA polymorphisms in tomato varieties revealedby InDel and SSR markers |
|
SHEN Lu1, SHEN Huo-lin1, CHAI Min2, WANG Yin-lei1, YANG Wen-cai1
|
| (1.College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,China;2.Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China) |
| Abstract: |
| The emergency of genomic sequences in tomato (Solanum lycopersicum L.) provides a base for developing InDel markers.In this study,18 InDel and 22 SSR markers were used to analyze genomic DNA diversity in 59 Chinese and 23 US fresh-market tomato varieties to investigate the potential use of InDel markers in tomato molecular breeding.Comparison between 59 Chinese and 23 US varieties were also conducted at marker level.The results indicated that InDel marker showed lower polymorphisms than SSR markers in 82 varieties.The number of alleles amplified by InDel primers was significantly lower than that amplified by SSR markers in Chinese varieties.But no obvious difference was observed in US varieties.The number of alleles amplified by InDel primers was significantly lower in Chinese varieties than in US varieties.Cluster analysis indicated that genetic difference was very low among varieties developed by Chinese breeding programs but relatively high between Chinese and foreign varieties. |
| Key words: Solanum lycopersicum InDel SSR marker polymorphism genomic DNA diversity |
