| 摘要: |
| 为筛选常温高效产酶复合菌系,分别以枯叶土壤、牛粪、堆肥和自然腐烂的小麦秸秆为微生物源,通过"外淘汰法"筛选了4组常温分解小麦秸秆并产胞外酶的复合菌系WSD-5、N、M和D。培养15 d后,WSD-5分解小麦秸秆75.6 %,而N、M和D的分解率分别为65.0 %、69.4 %和67.5 %。通过各复合菌系对小麦秸秆纤维素、半纤维素和木质素成分的分解比较,可知WSD-5复合菌系具有明显的分解优势:相对于初始秸秆,WSD-5复合菌系使麦秆中的纤维素、半纤维素和木质素分别减少了94.2 %、81.9 %和21.3 %。4组复合菌系虽然均检测到纤维素酶、木聚糖酶及滤纸酶的酶活性,但以WSD-5复合菌系的酶活性为最高。其中滤纸酶活性最高为1.30 U/mL,纤维素内切酶活性达4.35 U/mL,外切酶活性达到0.60 U/mL,β葡萄糖甘酶活性达到0.43 U/mL,木聚糖酶活性达到15.16 U/mL。特异引物PCR结果显示, WSD-5复合菌系由真菌和细菌共同组成。 |
| 关键词: 木质纤维素 微生物 分解菌复合系 酶活性 |
| DOI：10.11841/j.issn.1007-4333.2011.01.004 |
| 投稿时间：2010-09-16 |
| 基金项目:国家"十一五"科技支撑计划(2006BAD07A01) |
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| Comparison of four microbial communities with wheatstraw-degradation capability at normal temperature |
|
WANG Xiao-juan, LI Pei-pei, YUAN Xu-feng, WANG Xiao-fen, CUI Zun-jun
|
| (College of Agronomy and Biotechnology/Center of Biomass Engineering,China Agricultural University,Beijing 100193,China) |
| Abstract: |
| Four microbial communities named WSD-5, N,M and D,which with stable wheat straw-degradation capability,were constructed at normal temperature using the restricted cultivation technology.Compared with other three groups,WSD-5 exhibited the potential higher wheat straw-degradation ability during the process of continuous cultivation.On the 15th day,WSD-5 displayed the maximum wheat straw-degradation rate up to 75.6%,compared to N(65.0%),M(69.4%) and D(67.5%).Based on the results,up to 94.2% of cellulose,81.9% of hemicellulose,and 21.3% of lignin could be decomposed by WSD-5.The activities of cellulase,xylanase,and filter paper were detected for all the four communities and the results demonstrated that WSD-5 showed the highest enzyme activity (filter paper activity,1.30 U/mL;cellulose endonuclease activity,4.35 U/mL;exonuclease,0.60 U/mL;β-glucosidase activity,0.43 U/mL;xylanase activity,15.16 U/mL).The result of specific primer PCR showed that WSD-5 was consisted of fungi and bacteria together. |
| Key words: lignocellulose microbe degrading community enzyme activity |
