| 摘要: |
| 以9个甜高粱材料为对象,设0、70、140和210 mmol/L 4个NaCl浓度,在人工气候箱中进行发芽试验,分析了不同NaCl浓度对甜高粱发芽的影响,利用相对盐害率的隶属函数值综合评价了耐盐性;用聚丙烯酰胺凝胶垂直板电泳法,研究了不同NaCl浓度对甜高粱苗中POD同工酶的影响。结果表明:盐胁迫下甜高粱发芽率、发芽指数、发芽势、活力指数、芽长、根长、芽鲜质量、根鲜质量均与对照差异显著(P＜0.05)。耐盐性强弱顺序为:泰斯＞甜132＞BJK156＞考利＞吉甜3＞威利＞凯勒＞戴尔＞吉甜2。与对照相比,随着NaCl浓度增加,9个甜高粱材料POD同工酶表达量均逐渐增加,并且威利、甜132、凯勒、吉甜2、泰斯、吉甜3、考利在3个NaCl浓度下均诱导出了新的酶带P1,戴尔在140和210 mmol/L浓度下诱导出了新的酶带P1,BJK156仅在210 mmol/L浓度下诱导出了新的酶带P1,吉甜3在70 mmol/L浓度下酶带P4 消失,在140和210 mmol/L浓度下又逐渐出现。甜高粱POD同工酶谱的变化与其耐盐性强弱在一定程度上相关。 |
| 关键词: 甜高粱 NaCl 萌发 过氧化物同工酶(POD) |
| DOI：10.11841/j.issn.1007-4333.2011.01.003 |
| 投稿时间：2010-02-26 |
| 基金项目:科技部"十一五"科技支撑计划项目(2006BAD07A04,2009BADA7B01); 公益性行业(农业)科研专项经费项目(3-30-1) |
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| Effect of NaCl on germinating and expression ofperoxidase isozyme of sweet sorghum |
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WANG Xiu-ling1, CHEN Xu2, XIE Guang-hui2, LI Gui-ying3, ZHAO Wei-hua3
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| (1.College of Resources and Environmental Sciences,China Agricultural University,Beijing 100193,China;2.College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,China;3.Institution of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China) |
| Abstract: |
| Nine sweet sorghum cultivars were observed for salinity tolerance in climate incubator and irrigated with four NaCl concentrations of 0、70、140 and 210 mmol/L with three replications.Comprehensive evaluation on salt tolerance was studied by fuzzy mathematics.Effects of salt stress on POD isozyme of sweet sorghum seeding were studied by PAGE.As a result,there are significant (P＜0.05) differences between all treatments and control in germination rates,germination index,germination energy,bud length,root length,bud fresh weight,root fresh weight.Moreover,averages of the above eight indicators decrease along with the increase of salt concentrations.The order of salt-tolerance for nine varieties was:Thesi＞Tian132＞BJK156＞Cowley＞Jitian3＞Wray＞Keller＞Dale＞Jitian2.In the meantime,Pattern of POD isozyme bands became stronger and stronger with increase of salinity concentration,a novel isozyme band (P1) was induced at 210 mmol/L NaCl for BJK156,at 140,210 mmol/L NaCl for Dale,under all salt stress for Wray,Tian132,Keller,Jitian2,Thesi,Jitian3,Cowley.The isozyme band P4 disappeared at 70 mmol/L and appeared at 140,210 mmol/L NaCl for Jitian3.The changes of peroxidase isoenzyme profiles may be related to the salt tolerance in sweet sorghum. |
| Key words: sweet sorghum NaCl germinate peroxidase isozyme (POD) |
