| 摘要: |
| 为探索皖西白鹅繁殖周期中不同状态下垂体FSHβ mRNA的表达特性,选取开产前期(220日龄)、产蛋期、就巢起始期、就巢中后期和就巢后休产期的皖西白鹅母鹅各6只,采用荧光定量PCR方法分别测定了各时期垂体FSHβ mRNA的表达水平。结果显示,产蛋期母鹅垂体FSHβ mRNA表达水平为1 862.9±350.5,极显著高于其他时期(P<0.01)。产蛋前、就巢起始、就巢中后期以及就巢后休产期母鹅垂体FSHβ mRNA表达水平分别为95.5±33.8、248.2±40.0、122.3±41.3和211.6±56.8,但各时期间的表达水平差异不显著(P>0.05)。由此可见,皖西白鹅垂体FSHβ mRNA表达水平与繁殖周期密切相关,且产蛋期该基因表达水平的大幅度下调与就巢行为起始和维持有关。 |
| 关键词: 白鹅 繁殖周期 就巢 FSHβ mRNA |
| DOI：10.11841/j.issn.1007-4333.2010.06.014 |
| 投稿时间：2010-04-19 |
| 基金项目:国家科技支撑计划项目(2008BADC1B03)第一作者:姜润深,副教授,博士,主要从事家禽遗传育种研究,E-mail:jiangrunshen@ahau.edu.cn通讯作者:耿照玉,教授,主要从事家禽遗传育种研究,E-mail:jrsahpoultry@sina.com |
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| Expression of pituitary FSHβ mRNA during reproduction cyclein Wanxi white goose |
|
JIANG Run-shen, DU Xiao-dong, CHEN Xing-yong, GENG Zhao-yu
|
| (College of Animal Science and Technology,Anhui Agricultural University,Hefei 230036,China) |
| Abstract: |
| To investigate the expression of pituitary FSHβ mRNA at different stages during reproduction cycle in Wanxi white goose,the pituitary FSHβ mRNA level was tested by FQRT-PCR method in six female geese at stages of pre-laying,laying,onset of incubation,middle or later period of incubation,and laying cessation after incubation,respectively.Results showed that,the level of pituitary FSHβ mRNA (1 862.9±350.5) in laying stage was higher (P<0.01) than which at any of other stages.The average levels of pituitary FSHβ mRNA were 95.5±33.8,248.2±40.0,122.3±41.3,and 211.6±56.8 at pre-laying,onset of incubation,middle or later period of incubation,and laying cessation after incubation,respectively,but there is no significantly differ among them (P>0.05).Therefore,the level of pituitary FSHβ mRNA is highly associated with the reproduction cycle in Wanxi white goose,and a sharp decrease of pituitary FSHβ mRNA level in laying period associated with the onset and maintenance of incubation. |
| Key words: white goose reproduction cycle incubation FSHβ mRNA |
