| 摘要: |
| 为研究慢性冷应激对西门塔尔杂交犊牛免疫相关指标的影响,分别对4~6月龄和8~10月龄西门塔尔杂交犊牛在舍内平均温度为9.6、3.5、1.5、－1.1 和－2.4 ℃下采血,测量血清中生理生化指标、免疫相关激素及抗氧化指标。结果显示:随着温度的降低,试验组犊牛血清葡萄糖(BG)水平升高了18.8%~103.3%,在接受0 ℃左右(1.5和－1.1 ℃) 慢性冷应激时,BG的升高随着应激时间的延长而更加显著(P＜0.05),但温度对血清尿素氮(BUN)和血清皮质酮(CORT)指标的影响并不显著(P＞0.05)。舍内－4.0 ℃日平均温度下,显著提高了血清总抗氧化能力(T-AOC)和丙二醛(MDA)的水平、降低了超氧化物歧酶(SOD)和谷胱甘肽过氧化物酶(GSH)的水平(P＜0.05)。在－2.4 ℃日平均温度下,8~10月龄组的血清SOD水平比4~6月龄组高82.6%(P＜0.05),生长激素(GH)水平比4~6月龄组低26.0%(P＜0.05)。结果表明,慢性冷应激改变了血清中生理生化指标、免疫相关激素和抗氧化指标的水平,从而影响犊牛的免疫能力,并且温度越低、月龄越小,这种影响越大。 |
| 关键词: 犊牛 冷应激 血清 免疫 抗氧化 |
| DOI：10.11841/j.issn.1007-4333.2010.06.011 |
| 投稿时间：2010-04-12 |
| 基金项目:国家现代农业(肉牛)产业技术体系专项(nycytx-38); 公益性农业(肉牛)行业专项(nyhyzx07-035); 动物营养国家重点实验室项目第一作者:孟祥坤,硕士研究生,E-mail:mengxiangkun_@163.com通讯作者:曹兵海,教授,主要从事肉牛饲料营养与牛肉品质研究,E-mail:caobhchina@yahoo.com.cn |
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| Effects of chronic cold stress on immune ofSimmental crossbred calves |
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MENG Xiang-kun, CAO Bing-hai, ZHUANG Hong, WANG Mao, LI Teng
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| (National Beef Modern Industrial Technology R & D Center/College of Animal Science and Technology,China Agricultural University,Beijing 100193,China) |
| Abstract: |
| The experiment was conducted to study the effects of chronic cold stress on immune of Simmental crossbred calves (4－6 months group and 8－10 months group).Blood was collected at average temperature of 9.6,3.5,1.5,－1.1 and －2.4 ℃ indoor.Physiological and biochemical characteristics,hormone related to immune and antioxidative ability of serum were determined.The results showed that the level of BG in experimental group increased around 18.8% to 103.3% with the temperature decreasing (P<0.05),and the level of BG significantly enhanced under chronic cold stress as the tolerant of cold became longer (P<0.05).No differences observed in BUN and CORT (P>0.05).The level of T-AOC and MDA in serum significantly enhanced whereas SOD and GSH decreased (P<0.05) under chronic cold stress.At －2.4 ℃,the level of SOD in 8－10 months group increased by 82.6% compare with that of 4－6 months group,and level of GH in 8－10 months group reduced by 26.0% compare with that of 4－6 months group (P<0.05).The conclusion is that the levels of the physiological and biochemical characteristics,hormone related to immune and antioxidative ability of serum can be changed under chronic cold stress;and therefore affected the immunity ability of calves.It also showed that the lower temperature and the younger calves,the more remarkable they effects. |
| Key words: calves cold stress serum immunity antioxidant |
