| 摘要: |
| 为了解决甘蓝遇低温先期抽薹的难题,本试验采用37 ℃高温进行春化逆转,以低温春化的植株作为对照,探讨甘蓝体内蛋白酶活性与春化逆转的关系。甘蓝早熟品种"8398"和中熟品种"京丰"经春化逆转,可溶性蛋白含量、硝酸还原酶(NR)、过氧化物酶(POD)和过氧化氢酶(CAT)活性比正常春化的植株分别下降了13.1和6.6 mg/g,26.5和45.9 μg/(g·h),19.8和17.1 μg/(g·min),11.6和9.6 U/(g·min)。试验发现,早熟品种"8398" 其体内可溶性蛋白含量、POD、CAT活性的降低幅度均比中熟品种"京丰"更大些;而中熟品种"京丰"体内NR活性比早熟品种"8398"活性强。结果表明,低水平的可溶性蛋白含量、POD、CAT、NR活性有利于春化逆转的完成。 |
| 关键词: 甘蓝 春化逆转 可溶性蛋白 硝酸还原酶 过氧化物酶 过氧化氢酶 |
| DOI：10.11841/j.issn.1007-4333.2010.06.007 |
| 投稿时间：2010-04-26 |
| 基金项目:东北农业大学创新团队课题(CXT002-2-2); 黑龙江省教育厅寒地蔬菜生物学重点实验室项目第一作者:李文平,硕士研究生, E-mail:Leewenping@163.com通讯作者:于锡宏,教授,主要从事植物生长发育与设施园艺研究,E-mail:yxh100@sohu.com蒋欣梅,副研究员,主要从事植物生长发育与设施园艺研究,E-mail: jxm0917@163.com |
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| Relations between changes of protease in cabbagewith the devernalization |
|
LI Wen-ping, YU Xi-hong, JIANG Xin-mei, ZHANG Yu, LIU Dong
|
| (College of Horticulture,Northeast Agricultural University,Harbin 150030,China) |
| Abstract: |
| In order to solve the problem of cabbage early-bolting under low temperature,the relations between changes of protease in cabbage with the devernalization was investigated.Temperature at 37 ℃ was used for devernalization as a treatment,whereas low temperature vernalization of plants as a control.The content of soluble protein and the activity of NR,POD,CAT reduced by 13.1 and 6.6 mg/g,26.5 and 45.9 μg/(g·h),19.8 and 17.1 μg/(g·min),11.6 and 9.6 U/(g·min) for the early maturing variety of cabbage '8398’,and for the mid-variety of Cabbage 'Jingfeng’.The study found that the decline of the content of soluble protein,and the activity of POD,CAT in '8398’ was higher than that in 'Jingfeng’;but the activity of NR in 'Jing feng’ was higher than that in'8398’,the results showed that soluble protein contents,POD,CAT and NR activity at lower levels was benefit to devernalization. |
| Key words: cabbage devernalization soluble protein nitrate reductase peroxidase catalase |
