| 摘要: |
| 从传统发酵食品中分离筛选高产γ-氨基丁酸(GABA)的乳酸菌。采用薄层层析法和高效液相色谱法对分离出的82株乳酸菌的GABA生产能力进行定性和定量分析,筛选出1株高产GABA的乳酸菌S35,其产量达到4.52 g/L。经生理生化鉴定、API试剂鉴定和16S rRNA序列分析,菌株S35为植物乳杆菌。 |
| 关键词: 发酵食品 γ-氨基丁酸 植物乳杆菌 薄层层析 高效液相色谱 |
| DOI：10.11841/j.issn.1007-4333.2010.05.017 |
| 投稿时间：2010-02-25 |
| 基金项目:北京市优秀人才培养资助项目(20081D0105500030); 北京市自然科学基金资助项目(6093022); 北京市优秀人才资助计划(PYZZ091021001930)第一作者:渠岩,硕士研究生,E-mail:quyan_0220@163.com通讯作者:李平兰,教授,博士生导师,主要从事益生菌研究,E-mail:plli@sohu.com |
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| Screening and identification of γ-amino butyric acid-producingLactobacillus plantarum S35 from fermented food |
|
QU Yan1, LI Ping-lan1, WANG Fu-jie2, WANG Yang1
|
| (1.College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China;2.Beijing Food Brewing Institute,Beijing 100050,China) |
| Abstract: |
| This study focused on screening lactic acid bacteria with high production capacity of γ-amino butyric acid (GABA) from traditional fermented food.The GABA production of 82 lactic acid bacteria strains was measured by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC).The strain S35 with high production capacity of GABA was screened and its GABA yield reached 4.52 g/L.Finally,the strain S35 was identified as Lactobacillus plantarum by identification of physical,biochemical and identification of API 50 CHL and 16S rRNA sequence. |
| Key words: fermented food γ-amino butyric acid (GABA) Lactobacillus plantarum TLC HPLC |
