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毛樱桃对花生根结线虫的抗性研究
王衍纯1, 叶航2, 李辉1, 贾克功1, 朱立新1, 王仙林1
0
(1.中国农业大学 农业与生物技术学院,北京 100193;2.广西林业科学院,南宁 530006)
摘要:
以实生钵苗为试材,采用人工接种、根系次氯酸钠-酸性品红染色等方法研究了毛樱桃对花生根结线虫的抗性。结果表明:花生根结线虫的接种侵入率1.4%,根中雌成虫数量占接种量的0.3%,依据有无根结和雌成虫数量占接种线虫数量的百分比判定其高抗花生根结线虫;毛樱桃实生群体对花生根结线虫的抗性具有显著的株间分离现象,存在免疫、高抗和中抗3种抗性类型,3种抗性类型个体分别占群体总量的5.0%、86.7%和8.3%。毛樱桃对花生根结线虫具有极强的抗侵入与抗发育能力,是优异的抗花生根结线虫桃树砧木种质资源。
关键词:  毛樱桃  花生根结线虫  抗性评价  抗性分布  抗性机制
DOI:10.11841/j.issn.1007-4333.2010.05.012
投稿时间:2010-03-03
基金项目:引进国际先进农业科学技术项目(948)(201035);国家科技支撑计划项目(2007BAD57B05-4)第一作者:王衍纯,硕士研究生,E-mail:wangdundj@yahoo.cn通讯作者:贾克功,教授,主要从事桃树栽培理论与新技术研究,E-mail:jkgong@cau.edu.cn朱立新,副教授,主要从事核果类果树砧木研究,E-mail:yyzzl@cau.edu.cn
Resistance of Prunus tomentosa Thumb to Meloidogyne arenaria
WANG Yan-chun1, YE Hang2, LI Hui1, JIA Ke-gong1, ZHU Li-xin1, WANG Xian-lin1
(1.College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,China;2.Guangxi Forestry Reaserch Institute,Nanning 530006,China)
Abstract:
By using pot plantlets as test materials,the resistance of Prunus tomentosa Thumb to Meloidogyne arenaria was studied via artificial inoculation and staining method of acidic fuchsin.Results showed that:1) 1.4% second stage juvenile nematodes (J2) penetrated and 0.3% of them developed to female nematodes in all 1 000 inoculation J2 per plantlet;2) P.tomentosa Thumb was highly resistant to M.arenaria according to the number of gall and female nematode;3)Resistance was segregated in seedlings;immuned,highly resistant and resistant genotypes were found in P.tomentosa Thumb,for which the proportions were 5.0%、86.7% and 8.3%,respectively.P.tomentosa Thumb was a good resistant species and germplasm resource for M.arenaria because it can resist the penetration and delay the development of juvenile nematodes.
Key words:  Prunus tomentosa Thumb  Meloidogyne arenaria  resistance evaluation  resistance genotype  resistance mechanism