| 摘要: |
| 研究玉米须多糖的化学成分及免疫活性,利用系统提取分离方法,得到玉米须粗多糖(CSCP),利用高效液相和气相色谱对CSCP的分子质量分布及单糖组成进行分析;通过小鼠体外脾细胞增殖对CSCP进行免疫活性研究。结果显示:CSCP中77.5%的多糖分子质量分布于1.0×105~5.0×105之间;CSCP的单糖组成为鼠李糖、木糖、阿拉伯糖、甘露糖、葡萄糖和半乳糖,其摩尔比为1.00∶1.36∶1.30∶0.66∶2.52∶1.60,其中葡萄糖所占比例最大;CSCP能够明显刺激小鼠脾细胞增殖,并呈现一定的量效关系。 |
| 关键词: 玉米 多糖 高效液相色谱 红外图谱解析 免疫活性 |
| DOI：10.11841/j.issn.1007-4333.2010.04.004 |
| 投稿时间：2009-12-16 |
| 基金项目:农业部科技专项(200903001-2-3); 北京市"十一五"科技攻关项目(D07060200880701)第一作者:贾亚敏,博士研究生,E-mail:zyx.1002@163.com通讯作者:郭玉海,教授,博士生导师,主要从事药用作物栽培加工研究,E-mail:yhguo@cau.edu.cn |
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| Physical and chemical properties of corn silk polysaccharideand its immunity activity |
|
JIA Ya-min, ZHU Yan-xia, GUO Yu-hai
|
| (College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,China) |
| Abstract: |
| In order to study the chemical composition and immune activity of corn silk,we applied a systematic extraction and separation method to obtain crude polysaccharide from corn silk (CSCP for short).HLPC and GC used to analysis the molecular weight and monosaccharide composition.Mouse splenocyte proliferation was used to determine the immune activity of CSCP.The results showed that 75% of the CSCP molecular weight was between the 1.0×105 and 5.0×105.CSCP contained rhamnose,xylose,arabinose,mannose,glucose and galactose and other monosaccharides.The molar ratios were 1.00∶1.36∶1.30∶0.66∶2.52∶1.60.The largest proportion was glucose.CSCP significantly stimulated the proliferation of mouse spleen cells and had a definite dose-effect relationship. |
| Key words: corn polysaccharide high performance liquid chromatography IR spectra analysis immune activity |
