| 摘要: |
| 以皮下注射卵白蛋白(OVA)致敏的大鼠为模型,用甲苯胺蓝染色法和CD34免疫组化法,研究黄芪多糖粉针剂对大鼠皮肤组织内肥大细胞与微血管的影响。结果显示:皮下注射OVA刺激后,大鼠皮肤内CD34+微血管与肥大细胞密度分别从33.6和77.2个/mm2升高至67.2和180.0个/mm2,肥大细胞出现脱颗粒现象,且多聚集在微血管周围;黄芪多糖(APS)对OVA诱导的CD34+微血管与肥大细胞密度升高有显著下调作用,分别降至39.7和99.7个/mm2;且显著降低肥大细胞的脱颗粒现象。结果表明,通过下调微血管内皮细胞表面CD34的表达、减少肥大细胞在微血管旁的聚集与脱颗粒,从而抑制炎性细胞与微血管内皮细胞的黏附可能是黄芪多糖(APS)发挥抗炎作用的机制之一。 |
| 关键词: 黄芪多糖 肥大细胞 微血管 脱颗粒 |
| DOI：10.11841/j.issn.1007-4333.2010.01.012 |
| 投稿时间：2009-01-15 |
| 基金项目:北京市属市管高等学校人才强教计划资助项目;北京农学院三项基金重点项目(09-ZD-SY-04)第一作者:张爽,硕士研究生,E-mail:zhangshuang0122@yahoo.cn通讯作者:滕可导,教授,主要从事发育组织学研究,E-mail:kedao@cau.edu.cn |
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| Effect of astragalus polysaccharide powder injection on the density of microvessels and mast cells in ovalbulmin-sensitized rat skin |
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ZHANG Shuang1, ZHANG Tao2, TENG Ke-dao1, WANG An-ru1, YU Ying-fang1, ZHANG Han3
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| (1.College of Veterinary Medicine, China Agricultural University, Beijing 100193, China;2.Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, Beijing 102206, China;3.College of Life Science, Hebei University, Baoding 071002, China) |
| Abstract: |
| In order to investigate the effects of Astragalus polysaccharide (APS) on microvessels and mast cells, the experiment was based on the model of rat sensitized by subcutaneous injection of ovalbulmin (OVA), adopted CD34 immunochemistry method and toluidine blue staining to investigate the effects of APS on microvessels and mast cells in rat skin. After the stimulation of OVA, the densities of CD34+ microvessels and mast cells were significantly increased from 33.6 and 77.2 cells/mm2 to 67.2 and 180.0 cells/mm2, respectively, and the mast cell degranulation occurred around the microvessels. The APS powder injection significantly downregulated the density increase of CD34+ microvessels and mast cells to 39.7 and 99.7 cells/mm2 induced by OVA in rat skin, and decreased the degranulation of mast cells. It is one mechanism of anti-Inflammatory effect of APS that it downregulates the expression of CD34 in microvascular endothelial cells and the increase and degranulation of mast cells around microvessels, which maybe contribute to the adherence reducing between inflammatory cells and microvascular endothelial cells. |
| Key words: astragalus polysaccharide mast cell microvessel degranuation |
