| 摘要: |
| 用昼夜变温(20 ℃/10 ℃) -低温(4 ℃)、暖温(20 ℃) -低温(4 ℃)和低温(10 ℃) -低温(4 ℃)层积两阶段处理方法,研究变温层积处理过程中东北红豆杉种子生理生化特性的变化规律。结果表明:昼夜变温层积6个月后种胚长度和宽度分别由层积前的1.52和0.47 mm,增加到1.95和0.61 mm;昼夜变温-低温层积后种子可溶性糖含量达到46.3 mg/g,显著高于层积前;粗脂肪含量比层积前减少了3.8 %;脱氢酶(TTC)活性持续增加,层积10个月后达到层积前的2倍多;葡萄糖六磷酸脱氢酶(G-6-PDH)活性在昼夜变温层积阶段下降,低温层积阶段上升;三磷酸腺苷(ATP)水平在昼夜变温层积过程中变化不大,而低温层积后ATP水平显著增加,达到层积前的3.9倍。昼夜变温-低温层积的种子裂口率为11.4 %,出苗率为14.7 %,显著高于暖温和低温处理,说明昼夜变温-低温处理效果优于暖温-低温和低温-低温处理。 |
| 关键词: 变温层积 东北红豆杉 种子 休眠 种胚发育 生理生化特性 |
| DOI：10.11841/j.issn.1007-4333.2010.01.007 |
| 投稿时间：2009-05-18 |
| 基金项目:国家自然科学基金资助项目(30570180);北京市科委绿色通道项目(D07060200880701)第一作者:廖云娇,硕士研究生,E-mail:liaoyunjiao@gmail.com通讯作者:董学会,副教授,博士,主要从事器官发育与次生代谢的调控研究,E-mail:xuehuidong@cau.edu.cn |
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| Effect of different temperature stratifications on physiological and biochemical characteristic and seedembryos development of Taxus cuspidata Sieb.et Zucc. |
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LIAO Yun-jiao, LI Xue, DONG Xue-hui
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| (College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China) |
| Abstract: |
| Taxus (Taxus cuspidata Sieb. et Zucc.) seed is of deep embryo dormancy. In this study Taxus seed was treated with three temperature stratifications of changing temperature of day and night (20 ℃/10 ℃,day/night)-cold (4 ℃),warm(20 ℃)-cold(4 ℃),cold(10 ℃)-cold(4 ℃). The results showed that the length and width of embryos treated for 6 months were increased from 1.52 and 0.47 mm to 1.95 and 0.61 mm respectively. The content of soluble sugar increased to 46.3 mg/g, but crude fat decreased by 3.8 %. Dehydrogenase activity (TTC) at 10 months was 2 times as high as that before treatment. The activity of glucose-6-phosphate dehydrogenas (G-6-PDH) decreased at anterior phase but increased at latter phase. Adenosine triphosphate (ATP) content was steady with a low level at the first phase, but remarkably increased at the second phase, 3.9 times higher than the initial value. The percentage of breached seeds and germination rate of changed temperature-cold stratification were 11.4 % and 14.7 % respectively, and significantly higher than that of warm-cold and cold-cold stratification. It is indicated that the changing temperature of day and night-cold stratification was more effective to break out seed dormancy than warm-cold and cold-cold stratification. |
| Key words: temperature change stratification Taxus (T.cuspidata Sieb. et Zucc.) seed dormancy embryos development physiological and biochemical characteristic |
