| 摘要: |
| 采用RT-PCR和RACE技术从短日照草莓品种'卡姆罗莎’(Fragaria×ananassa Duch. cv. 'Cama-rosa’)嫩叶中克隆得到与草莓成花相关的基因,命名为FaCO(GenBank登录号,FJ377616)。该基因cDNA全长1 475 bp,1 152 bp的开放阅读框编码一个含有384个氨基酸,分子质量为42 ku的蛋白。该蛋白具有典型的CO同源蛋白结构,包含B-box1, B-box2, CCT 结构域及COOH区域。序列分析结果表明,草莓CO蛋白与其他物种的CO蛋白具有较高的同源性,其中与杨树(Populus deltoids)的CO蛋白同源性最高,差异为80%。构建pGEX-4T-1/FaCO原核表达体系,获得了分子质量为60 ku的融合蛋白。 |
| 关键词: CONSTANS基因 草莓 克隆 表达 |
| DOI：10.11841/j.issn.1007-4333.2010.01.004 |
| 投稿时间：2009-04-22 |
| 基金项目:新世纪人才支持计划(NCET-06-0108); 国家"十一五"科技支撑计划(2007BAD36B02); 国家自然科学基金资助项目(30871696)第一作者:赵志琴,硕士研究生,E-mail:jjk421@163.com通讯作者:李天红,教授,博士,主要从事果树生理与分子生物学研究,E-mail: lth@cau.edu.cn |
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| Cloning and expression analysis of CONSTANS gene in strawberry |
|
ZHAO Zhi-qin, AN Li-jun, LI Fang, LI Tian-hong
|
| (College of Agronomy and Biotechnology/Key Laboratory of Stress Physiology and Molecular Biology for Tree Fruits of Beijing, China Agricultural University, Beijing 100193, China) |
| Abstract: |
| A CONSTANS homologous gene named FaCO was cloned from young leaves of strawberry (Fragaria ×ananassa Duch. cv. 'Camarosa’) by RT-PCR and RACE technology. The gene had a cDNA sequence with 1 475 bp in length and contained an open reading frame of 1 152 bp which coding a deduced 384 amino acids protein. The peptide had two B-boxes, a CCT domain and a COOH region shared highly homology with CO of other plants, especially with CO of Populus deltoids, to 80%. The pGEX-4T-1/FaCO prokaryotic expression systems were constructed and induced. SDS-PAGE analysis showed that the rFaCO molecular weight was about 60 ku. |
| Key words: CONSTANS gene Fragaria×ananassa cloning expression analysis |
